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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-20054 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Rad50 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAD50 Antibody [NBP2-20054] - Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE gel, diluted at 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad50 Antibody [NBP2-20054] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad50 Antibody [NBP2-20054] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal diluted at 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad50 Antibody [NBP2-20054] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [C1C2], Internal diluted at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Rad50 Antibody [NBP2-20054] - NT2D1 whole cell extract A. 50 ug NT2D1 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of Rad50 protein by 4 ug Rad50 antibody [C1C2], Internal 5 % SDS-PAGE The immunoprecipitated Rad50 protein was detected by Rad50 antibody [C1C2], Internal diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RAD50 Antibody [NBP2-20054] - Immunohistochemical analysis of paraffin-embedded Colon carcinoma, using antibody at 1:250 dilution.
