Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-20055 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Rad50 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAD50 Antibody [NBP2-20055] - Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE gel, diluted at 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RAD50 Antibody [NBP2-20055] - Rad50 antibody [N1N2], N-term detects RAD50 protein by Western blot analysis. A. 30 ug Neuro2A whole lysate/extract B. 30 ug GL261 whole cell lysate/extract5 % SDS-PAGERad50 antibody [N1N2], N-term dilution: 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad50 Antibody [NBP2-20055] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad50 Antibody [NBP2-20055] - Rad50 antibody immunoprecipitates Rad50 protein in IP experiments. IP samples: NT2D1 whole cell extract. A. 50 ug NT2D1 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of Rad50 protein by 4 ug Rad50 antibody. 5 % SDS-PAGE. The immunoprecipitated Rad50 protein was detected by Rad50 antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad50 Antibody [NBP2-20055] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad50 Antibody [NBP2-20055] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RAD50 Antibody [NBP2-20055] - Immunohistochemical analysis of paraffin-embedded Lung carcinoma, using antibody at 1:250 dilution.