MA1-23269

antibody from Invitrogen Antibodies

Targeting: RAD50 hRad50, RAD50-2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-23269

Provider

Invitrogen Antibodies

Product name

RAD50 Monoclonal Antibody (13B3)

Antibody type

Monoclonal

Antigen

Other

Description

Recommended positive controls: HeLa, HeLa nuclear extract.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

13B3

Vial size

100 µL

Concentration

1.7 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Loss of tumor suppressor inositol polyphosphate 4-phosphatase type B impairs DNA double-strand break repair by destabilization of DNA tethering protein Rad50.

Sun Y, Ning X, Fan J, Hu J, Jiang Y, Hu Z, Paulo JA, Liu J, Qiu X, Xu H, Fu S, Gygi SP, Zhang J, Zhou C
Cell death & disease 2020 Apr 27;11(4):292

---

Positional stability of single double-strand breaks in mammalian cells.

Soutoglou E, Dorn JF, Sengupta K, Jasin M, Nussenzweig A, Ried T, Danuser G, Misteli T
Nature cell biology 2007 Jun;9(6):675-82

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RAD50 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RAD50 Monoclonal Antibody (13B3) (Product # MA1-23269) diluted at 1:200. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 Interaction of INPP4B with Rad50 and destabilization of Rad50 upon loss of INPP4B. a Rad50 was identified from immuno-precipitated INPP4B complexes by mass spectrometry. b Reciprocal verification of the interaction between INPP4B and Rad50 in A549 cells. Two micrograms of antibodies against INPP4B or Rad50 were used for each reciprocal immunoprecipitation in a total of 2 mg of A549 cell lysates, and the precipitated proteins were resolved on 6% SDS-PAGE gel and detected with the indicated antibodies. In IR treatments, cells were harvested at 2 h post IR. c Representative Western blot images for measuring the stability of Rad50 in CTL and Crispr-INPP4B A549 cells. The half-life of Rad50 in each cell line was determined by densitometry scanning compared to the signal intensity at time zero with CHX treatment, and normalized to GAPDH signal. The average half-life of Rad50 was based on three independent CHX treatments and plotted in d , and data are presented as means +- SD (min). An error bar represents SD. Asterisk (*) indicates p < 0.05 by Student's t -test.