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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [6]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX119731 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX119731, RRID:AB_10618353
- Product name
- Rad50 antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Activation of DNA damage repair pathways by murine polyomavirus.
Heiser K, Nicholas C, Garcea RL
Virology 2016 Oct;497:346-356
Virology 2016 Oct;497:346-356
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rad50 antibody [N1N2], N-term detects RAD50 protein by western blot analysis.A. 30 ?g Neuro2A whole lysate/extract B. 30 ?g GL261 whole cell lysate/extract5% SDS-PAGERad50 antibody [N1N2], N-term (GTX119731) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NT2D1 5% SDS PAGE GTX119731 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HeLa whole cell and nuclear extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rad50 antibody [N1N2], N-term immunoprecipitates Rad50 protein in IP experiments.IP samples: NT2D1 whole cell extractA. 50 £gg NT2D1 whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of Rad50 protein by 4 £gg Rad50 antibody [N1N2], N-term (GTX119731)5 % SDS-PAGEThe immunoprecipitated Rad50 protein was detected by Rad50 antibody [N1N2], N-term (GTX119731) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Rad50(GTX119731) antibody at 1:250 dilution.
