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- Antibody Data
- Antigen structure
- References [4]
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- Western blot [2]
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- Product number
- NB100-1487 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1487, RRID:AB_10001160
- Product name
- Rabbit Polyclonal Rad50 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references NBS1 is regulated by two kind of mechanisms: ATM-dependent complex formation with MRE11 and RAD50, and cell cycle-dependent degradation of protein.
Targeting Rad50 sensitizes human nasopharyngeal carcinoma cells to radiotherapy.
Increased oxidative stress in AOA3 cells disturbs ATM-dependent DNA damage responses.
Dimerization of the Rad50 protein is independent of the conserved hook domain.
Zhou H, Kawamura K, Yanagihara H, Kobayashi J, Zhang-Akiyama QM
Journal of radiation research 2017 Jul 1;58(4):487-494
Journal of radiation research 2017 Jul 1;58(4):487-494
Targeting Rad50 sensitizes human nasopharyngeal carcinoma cells to radiotherapy.
Chang L, Huang J, Wang K, Li J, Yan R, Zhu L, Ye J, Wu X, Zhuang S, Li D, Zhang G
BMC cancer 2016 Mar 7;16:190
BMC cancer 2016 Mar 7;16:190
Increased oxidative stress in AOA3 cells disturbs ATM-dependent DNA damage responses.
Kobayashi J, Saito Y, Okui M, Miwa N, Komatsu K
Mutation research. Genetic toxicology and environmental mutagenesis 2015 Apr;782:42-50
Mutation research. Genetic toxicology and environmental mutagenesis 2015 Apr;782:42-50
Dimerization of the Rad50 protein is independent of the conserved hook domain.
Cahill D, Carney JP
Mutagenesis 2007 Jul;22(4):269-74
Mutagenesis 2007 Jul;22(4):269-74
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Rad50 Antibody [NB100-1487] - Simple Western lane view shows a specific band for Rad50 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad50 Antibody [NB100-1487] - Detection of Rad50 using NB100-1487. Lane 1: 25 ng purified hMre11-hRad50 complex Lane 2: 25 ug crude HeLa extract.
