Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-16531 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAD50 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Suggested positive control: antigen standard for RAD50 (transient overexpression lysate).
- Reactivity
- Human, Mouse, Hamster
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- 4° C, do not freeze
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of Rad50 in HeLa nuclear extracts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAD50 in HeLa nuclear extracts. Sample was incubated in RAD50 polyclonal antibody (Product # PA1-16531).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAD50 in 0.5 mg/mL HeLa lysate. Samples were incubated in RAD50 polyclonal antibody (Product # PA1-16531). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Rad50 was performed by loading ~300,000 cell equivalents per lane in 1X LDS reducing sample buffer (Product # NP0007) and molecular weight ladder onto a 3-8% Tris-Acetate protein gel (Product # EA0375BOX). Proteins were transferred to a PVDF membrane at 100 mA overnight. Membrane was blocked in 5% milk/TBST for 1 hour. Rad50 was detected using a polyclonal antibody (Product # PA1-16531) diluted 1:2000 at 4°C, overnight. Membrane was then probed with anti-rabbit secondary antibody at a dilution of 1:5000. Chemiluminescent detection was performed using Pierce ECL western blot substrate (Product # 32106). Data courtesy of the Antibody Data Exchange Program.
