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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-31555 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAD50 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, mouse testis, NT2D1, rat testis.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RAD50 was performed by separating 50 µg of mouse tissue extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD50 Polyclonal Antibody (Product # PA5-31555) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RAD50 Polyclonal Antibody (Product # PA5-31555). Sample (30 µg of whole cell lysate). Lane A: NT2D1. 5% SDS PAGE. RAD50 Polyclonal Antibody (Product # PA5-31555) diluted at 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RAD50 was performed by separating 50 µg of rat tissue extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD50 Polyclonal Antibody (Product # PA5-31555) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Rad50 (Product # PA5-31555) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAD50 Polyclonal Antibody immunoprecipitates Rad50 protein in IP experiments. IP samples: NT2D1 whole cell extract. A. 50 µg NT2D1 whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of Rad50 protein by 4 µg RAD50 Polyclonal Antibody (Product # PA5-31555). 5 % SDS-PAGE. The immunoprecipitated Rad50 protein was detected by RAD50 Polyclonal Antibody (Product # PA5-31555) diluted at 1:500.
