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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 600-401-996 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-996, RRID:AB_2229200
- Product name
- Anti-Cyclin-Dependent Kinase 9 (CDK9) pT29 [RABBIT] Antibody - 600-401-996
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-CDK9 pT29 antibody shows detection of phosphorylated CDK9. 100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29. The primary antibody was used at a 1:1000 dilution. Personal Communication, J. Brady, NCI, Bethesda, MD.
- Validation comment
- Western Blot
