PA5-21329

antibody from Invitrogen Antibodies

Targeting: MUC2

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21329

Provider

Invitrogen Antibodies

Product name

MUC2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: . Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Loss of H2R Signaling Disrupts Neutrophil Homeostasis and Promotes Inflammation-Associated Colonic Tumorigenesis in Mice.

Shi Z, Mori-Akiyama Y, Du W, Fultz R, Zhao Y, Ruan W, Venable S, Engevik MA, Versalovic J
Cellular and molecular gastroenterology and hepatology 2022;13(3):717-737

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MUC2 Polyclonal Antibody detects secreted MUC2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. MUC2 stained by MUC2 Polyclonal Antibody (Product # PA5-21329) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MUC2 Polyclonal Antibody detects secreted MUC2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. MUC2 stained by MUC2 Polyclonal Antibody (Product # PA5-21329) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of MUC2 was performed in paraffin-embedded human intestine tissue using MUC2 Polyclonal Antibody (Product # PA5-21329) at a dilution of 1:750.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of MUC2 was performed in paraffin-embedded mouse colon tissue using MUC2 Polyclonal Antibody (Product # PA5-21329) at a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of MUC2 was performed in paraffin-embedded mouse intestine tissue using MUC2 Polyclonal Antibody (Product # PA5-21329) at a dilution of 1:2000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 H2R inactivation in mice resulted in aggravated DSS-induced colitis. ( A ) H&E staining (magnification: 40x and 200x) of middle and distal colon of WT and H2R-deficient mice after DSS treatment. Crypt polarity and architecture were disrupted in the middle and distal colon of H2R-deficient mice. ( B ) Inflammation scores were calculated based on the epithelial damage and infiltrating inflammatory cells from proximal to distal colon in 3 H&E sections from each mouse (total, 6 pairs of mice). Values are means +- SD. ** P < .01. ( C ) Immunofluorescence (magnification: 40x, 200x, and 1000x) of S100A8 (green), proliferating cell nuclear antigen (PCNA) (red), and 4',6-diamidino-2-phenylindole (DAPI) (blue, nuclear DNA staining) in the DSS-treated colon in WT and H2R KO mice. ( D ) DSS-induced hyperplasia ( top , indicated by a black arrowhead ) and dysplasia ( bottom , indicated by white arrowheads ) (magnification: 40x) in the colons of WT and H2R KO mice, respectively. Immunofluorescence (magnification: 200x and 400x) of MUC2 (red) and PCNA (green) shows much fewer MUC2-positive mature goblet cells in the DSS-treated colon of H2R KO mice. ( E ) The incidence of squamous metaplasia of DSS-treated WT (n = 8) and H2R KO (n = 7) mice. ( F ) H&E staining (magnification: 40x and 200x) and immunofluorescence (magnification: 200x and 1000x) of S100A8 (green, indicated by a white arrow) and PCNA (red) for the DSS-induced SM lesions (indicated by a black arrow ) in the distal colo