Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [5]
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Validation data
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- Product number
- NB120-11197 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-11197, RRID:AB_791261
- Product name
- Mouse Monoclonal MUC2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. NB120-11197 recognizes the human MUC2 mucin, and shows no cross-reactivity with MUC1, MUC3 or MUC4 mucins.In tissue sections NB120-11197 recognizes colon, liver and prostate tissues strongly. The recognizes malignant colonic mucosa and normal mucosa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Inhibition of corticotropin-releasing hormone receptor 1 and activation of receptor 2 protect against colonic injury and promote epithelium repair.
Role of Janus kinase 3 in mucosal differentiation and predisposition to colitis.
Li B, Lee C, Filler T, Hock A, Wu RY, Li Q, Chen S, Koike Y, Ip W, Chi L, Zani-Ruttenstock E, Määttänen P, Gonska T, Delgado-Olguin P, Zani A, Sherman PM, Pierro A
Scientific reports 2017 May 11;7:46616
Scientific reports 2017 May 11;7:46616
Role of Janus kinase 3 in mucosal differentiation and predisposition to colitis.
Mishra J, Verma RK, Alpini G, Meng F, Kumar N
The Journal of biological chemistry 2013 Nov 1;288(44):31795-806
The Journal of biological chemistry 2013 Nov 1;288(44):31795-806
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Analysis of Allophycocyanin conjugate of NB120-11197. An intracellular stain was performed on HeLa cells with MUC2 (996/1) antibody NB120-11197APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were fixed with 4% PFA and then permeabli
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Permeabilised HL-60 cells stained with Mouse anti Human MUC-2:FITC
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - Analysis of Allophycocyanin conjugate of NB120-11197. An intracellular stain was performed on NTERA-2 cells with MUC2 (996/1) antibody NB120-11197APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were fixed with 4% PFA and then permea
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody (996/1) NB120-11197AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: MUC2 Antibody (996/1) [NB120-11197] - An intracellular stain was performed on HeLa cells with MUC2 Antibody [996/1] NB120-11197AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.