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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-31880 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NAPRT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116. Predicted reactivity: Mouse (85%), Rat (80%), Bovine (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references NAD metabolic dependency in cancer is shaped by gene amplification and enhancer remodelling.
NAMPT is the cellular target of STF-31-like small-molecule probes.
Chowdhry S, Zanca C, Rajkumar U, Koga T, Diao Y, Raviram R, Liu F, Turner K, Yang H, Brunk E, Bi J, Furnari F, Bafna V, Ren B, Mischel PS
Nature 2019 May;569(7757):570-575
Nature 2019 May;569(7757):570-575
NAMPT is the cellular target of STF-31-like small-molecule probes.
Adams DJ, Ito D, Rees MG, Seashore-Ludlow B, Puyang X, Ramos AH, Cheah JH, Clemons PA, Warmuth M, Zhu P, Shamji AF, Schreiber SL
ACS chemical biology 2014 Oct 17;9(10):2247-54
ACS chemical biology 2014 Oct 17;9(10):2247-54
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NAPRT1 in methanol-fixed HeLa cells using a NAPRT1 polyclonal antibody (Product # PA5-31880) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Nicotinic acid blocks the effect of STF-31 andother NAMPT inhibitors in NAPRT1-expressing cells. (A) HCT116 andHT1080 cells were tested for viability after 72 h treatment with compound146 and known NAMPT inhibitors in the presence or absence of 10 muMnicotinic acid (NA). Each data point is mean +- SD ( n = 3). (B) Sensitivity of HCT116 cells tranfected with control orNAPRT1 siRNA to compound 146 in the presence or absence of NA. Eachdata point is mean +- SD ( n = 3). (C) Sensitivity of HCT116 against compound 146in different media conditions. Each data point is mean ( n = 2). (D) NAPRT1 -dependent effects of nicotinicacid (NA) on STF-31 sensitivity across 772 CCLs. Sensitive cell lineswere defined as having an AUC < 3.5, and insensitive cell linesas an AUC > 5.5. Red bars = mean +- SD.
