Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- ALX-804-150-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-150-C100, RRID:AB_2051330
- Product name
- DR6 (human) monoclonal antibody (Luke-1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- Luke-1
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- HEK 293T cells were mock transfected (thin line) or transfected with an expression plasmid coding for the extracellular domain of human DR6 (thick line). Method: Cells (5x10E5) were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% FCS, 0.02% azide) containing 1µg/ml of anti-DR6 MAb (Luke-1). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.