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LearnMA5-24347
antibody from Invitrogen Antibodies
Targeting: HPGDS
GSTS, GSTS1, GSTS1-1, H-PGDS, PGD2, PGDS
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- MA5-24347 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HPGDS Monoclonal Antibody (735301)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 735301
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of NCI-H460 human large cell lung carcinoma cell line, human lung tissue, and human placenta tissue. PVDF membrane was probed with 0.25 µg/mL of mouse Anti-human Hematopoietic Prostaglandin D Synthase/HPGDS Monoclonal Antibody (Product # MA5-24347) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for Hematopoietic Prostaglandin D Synthase/HPGDS at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HPGDS in NCI‚H460 human large cell lung carcinoma cell line, human lung tissue, and human placenta tissue. Samples were incubated in HPGDS monoclonal antibody (Product # MA5-24347) using a dilution of 0.25 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for Hematopoietic Prostaglandin D Synthase/HPGDS at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of HPGDS in immersion fixed HEL 92.1.7 human erythroleukemic cell line. Samples were incubated in HPGDS monoclonal antibody (Product # MA5-24347) using a dilution of 8 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
