PA1-32129

antibody from Invitrogen Antibodies

Targeting: ABCA1 ABC1, HDLDT1, TGD

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Gel shift (Recommended by provider)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

PA1-32129

Provider

Invitrogen Antibodies

Product name

ABCA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian, Hamster, Porcine

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ABCA1 in HepG2 cells. Cells were fixed with 10% formalin, permeabilized with 0.5% Triton-X100 and ABCA1 Polyclonal Antibody (Product # PA1-32129) (Green) at a dilution of 5 µg/mL was used as a primary antibody. Red : Tubulin. Blue: DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ABCA1 in HepG2 cells(serum starvation 24hrs and treated with 1uM TO9). Cells were fixed with 10% formalin, permeabilized with 0.5% Triton-X100 and ABCA1 Polyclonal Antibody (Product # PA1-32129) (Green) at a dilution of 5 µg/mL was used as a primary antibody. Red : Tubulin. Blue: DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of ABCA1 in prostate tissue using ABCA1 Polyclonal Antibody (Product # PA1-32129).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of Glypican 3 was performed in HeLa cells using Glypican 3 Monoclonal Antibody (GT764) (Product # MA5-31547) (Purple) at a dilution of 1:400. M1 is defined by unstained cells.