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- Validations
- Western blot [1]
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- Product number
- PA5-22908 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ABCA1 Polyclonal Antibody, DyLight™ 488
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian, Hamster, Porcine, Rabbit
- Host
- Rabbit
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.65 mg/mL
- Storage
- 4° C, store in dark
Submitted references MEK1/2 Inhibition Promotes Macrophage Reparative Properties.
Long ME, Eddy WE, Gong KQ, Lovelace-Macon LL, McMahan RS, Charron J, Liles WC, Manicone AM
Journal of immunology (Baltimore, Md. : 1950) 2017 Jan 15;198(2):862-872
Journal of immunology (Baltimore, Md. : 1950) 2017 Jan 15;198(2):862-872
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ABCA1 was performed by loading 30 µg of HEK293T whole cell lysates per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat dry milk in TBST for 1 hour at room temperature. The membrane was probed with a DyLight 488-conjugated ABCA1 polyclonal antibody (Product # PA5-22908) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:40,000 for 1 hour at room temperature. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ABCA1 (green) in HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a DyLight 488-conjugated ABCA1 polyclonal antibody (Product # PA5-22908) at a dilution of 1:200 for 1 hour at room temperature in 1X PBS containing 1% BSA and 0.3% Triton X-100, and then washed with 1X PBS. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 at 40X magnification. Data courtesy of the Innovators Program.
- Conjugate
- Green dye
