PA5-22906

antibody from Invitrogen Antibodies

Targeting: ABCA1 ABC1, HDLDT1, TGD

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Blocking/Neutralizing (Recommended by provider)

Gel shift (Recommended by provider)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22906

Provider

Invitrogen Antibodies

Product name

ABCA1 Polyclonal Antibody, Biotin

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian, Hamster, Porcine, Rabbit

Host

Rabbit

Conjugate

Biotin

Isotype

IgG

Vial size

100 μL

Concentration

0.81 mg/mL

Storage

4°C, store in dark

References

Submitted references

Dysregulated Lipid Transport Proteins Correlate With Pathogenesis and Outcome in Severe Alcoholic Hepatitis.

Maras JS, Das S, Bhat A, Kumar Vyas A, Yadav G, Chaudhary S, Sukriti S, Gupta AC, Bihari C, Mahiwall R, Sarin SK
Hepatology communications 2019 Dec;3(12):1598-1625

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Quantitative proteomic analysis identifies significantly down-regulated lipid transporter proteins in SAH. (A) Partial list of proteins differentially regulated (>1.5-fold; P < 0.05) between SAH, AC, HC, and its GO analysis, which documents enrichment of lipid transporters in SAH. (B) KEGG enrichment analysis of the up-regulated (n = 32) and down-regulated (n = 176) proteins in patients with SAH represents significant ( P < 0.05) enrichment. (C) Plasma level of PON1 was down-regulated in patients with SAH (median, 23.2; range, 2.3-139.4) compared to those with AC (median, 44.7; range, 25.8-131) and HC (median, 139.5; range, 46.6-196.0) ( P < 0.05). (D) Plasma level of apolipoproteins (APOB, APOE, APOA1, APOA2, APOC1, APOC3) in SAH compared to AC and HC ( P < 0.05 is significant). (E) IHC showing expression of PON1, LDL, CD36, SRA1, ABCA1, and ABCG1 in SAH (n = 5) and AC (n = 5). For all IHC analyses, relative quantization of positively stained cells are expressed as mean number of positive cells/10 high-power field (40x) and * P < 0.05. Abbreviations: COA, coenzyme A; HBG1, hemoglobin subunit gamma 1; KEGG, Kyoto Encyclopedia of Genes and Genomes; LBP, lipopolysaccharide binding protein; LPS, lipopolysaccharide; TLR4, toll-like receptor 4;

Conjugate

Biotin