NB400-164

antibody from Novus Biologicals

Targeting: ABCA1 ABC1, HDLDT1, TGD

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB400-164

Provider

Novus Biologicals

Proper citation

Novus Cat#NB400-164, RRID:AB_10000730

Product name

Rat Monoclonal ABCA1 Antibody

Antibody type

Monoclonal

Description

Unpurified.

Reactivity

Human, Mouse

Host

Rat

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Ovarian cholesterol efflux: ATP-binding cassette transporters and follicular fluid HDL regulate cholesterol content in mouse oocytes†.

Quiroz A, Molina P, Santander N, Gallardo D, Rigotti A, Busso D
Biology of reproduction 2020 Feb 14;102(2):348-361

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Sphingosine kinase-2 prevents macrophage cholesterol accumulation and atherosclerosis by stimulating autophagic lipid degradation.

Ishimaru K, Yoshioka K, Kano K, Kurano M, Saigusa D, Aoki J, Yatomi Y, Takuwa N, Okamoto Y, Proia RL, Takuwa Y
Scientific reports 2019 Dec 4;9(1):18329

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LRP1 integrates murine macrophage cholesterol homeostasis and inflammatory responses in atherosclerosis.

Xian X, Ding Y, Dieckmann M, Zhou L, Plattner F, Liu M, Parks JS, Hammer RE, Boucher P, Tsai S, Herz J
eLife 2017 Nov 16;6

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Age-Dependent Regulation of the Blood-Brain Barrier Influx/Efflux Equilibrium of Amyloid-β Peptide in a Mouse Model of Alzheimer's Disease (3xTg-AD).

Do TM, Dodacki A, Alata W, Calon F, Nicolic S, Scherrmann JM, Farinotti R, Bourasset F
Journal of Alzheimer's disease : JAD 2016;49(2):287-300

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Inhibition of inflammatory signaling pathways in 3T3-L1 adipocytes by apolipoprotein A-I.

Sultana A, Cochran BJ, Tabet F, Patel M, Torres LC, Barter PJ, Rye KA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2016 Jun;30(6):2324-35

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ABCG2- and ABCG4-mediated efflux of amyloid-β peptide 1-40 at the mouse blood-brain barrier.

Do TM, Noel-Hudson MS, Ribes S, Besengez C, Smirnova M, Cisternino S, Buyse M, Calon F, Chimini G, Chacun H, Scherrmann JM, Farinotti R, Bourasset F
Journal of Alzheimer's disease : JAD 2012;30(1):155-66

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Direct evidence of abca1-mediated efflux of cholesterol at the mouse blood-brain barrier.

Do TM, Ouellet M, Calon F, Chimini G, Chacun H, Farinotti R, Bourasset F
Molecular and cellular biochemistry 2011 Nov;357(1-2):397-404

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Sphingosine-1-phosphate receptor-2 deficiency leads to inhibition of macrophage proinflammatory activities and atherosclerosis in apoE-deficient mice.

Wang F, Okamoto Y, Inoki I, Yoshioka K, Du W, Qi X, Takuwa N, Gonda K, Yamamoto Y, Ohkawa R, Nishiuchi T, Sugimoto N, Yatomi Y, Mitsumori K, Asano M, Kinoshita M, Takuwa Y
The Journal of clinical investigation 2010 Nov;120(11):3979-95

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LRP1 controls cPLA2 phosphorylation, ABCA1 expression and cellular cholesterol export.

Zhou L, Choi HY, Li WP, Xu F, Herz J
PloS one 2009 Aug 31;4(8):e6853

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Detection of ABCA1 using NB400-164 at various dilutions in ABCA1 transfected HeLa lysates.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ABCA1 Antibody (3A1.891.3) [NB400-164] - Analysis of ABCA1 in (A) T09 treated RAW 264.7 cells and (B) untreated RAW 264.7 cells.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ABCA1 Antibody (3A1.891.3) [NB400-164] - Analysis of FFPE mouse liver using ABCA1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Plasma membrane staining was observed. Staining was performed by Histowiz.