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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- NB100-2068 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2068, RRID:AB_10002789
- Product name
- Mouse Monoclonal ABCA1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references 7-Ketocholesterol and cholestane-triol increase expression of SMO and LXRα signaling pathways in a human breast cancer cell line.
PMP22 Regulates Cholesterol Trafficking and ABCA1-Mediated Cholesterol Efflux.
Angiotensin II induces cholesterol accumulation and injury in podocytes.
Inhibition of macrophage oxidative stress prevents the reduction of ABCA-1 transporter induced by advanced glycated albumin.
Targeted disruption of the idol gene alters cellular regulation of the low-density lipoprotein receptor by sterols and liver x receptor agonists.
Protective effects of the citrus flavanones to PC12 cells against cytotoxicity induced by hydrogen peroxide.
Cyclosporine stimulates the renal epithelial sodium channel by elevating cholesterol.
Overexpression of ABCA1 reduces amyloid deposition in the PDAPP mouse model of Alzheimer disease.
Levy D, de Melo TC, Oliveira BA, Paz JL, de Freitas FA, Reichert CO, Rodrigues A, Bydlowski SP
Biochemistry and biophysics reports 2019 Sep;19:100604
Biochemistry and biophysics reports 2019 Sep;19:100604
PMP22 Regulates Cholesterol Trafficking and ABCA1-Mediated Cholesterol Efflux.
Zhou Y, Miles JR, Tavori H, Lin M, Khoshbouei H, Borchelt DR, Bazick H, Landreth GE, Lee S, Fazio S, Notterpek L
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Jul 3;39(27):5404-5418
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Jul 3;39(27):5404-5418
Angiotensin II induces cholesterol accumulation and injury in podocytes.
Yang Y, Yang Q, Yang J, Ma Y, Ding G
Scientific reports 2017 Sep 6;7(1):10672
Scientific reports 2017 Sep 6;7(1):10672
Inhibition of macrophage oxidative stress prevents the reduction of ABCA-1 transporter induced by advanced glycated albumin.
de Souza Pinto R, Castilho G, Paim BA, Machado-Lima A, Inada NM, Nakandakare ER, Vercesi AE, Passarelli M
Lipids 2012 May;47(5):443-50
Lipids 2012 May;47(5):443-50
Targeted disruption of the idol gene alters cellular regulation of the low-density lipoprotein receptor by sterols and liver x receptor agonists.
Scotti E, Hong C, Yoshinaga Y, Tu Y, Hu Y, Zelcer N, Boyadjian R, de Jong PJ, Young SG, Fong LG, Tontonoz P
Molecular and cellular biology 2011 May;31(9):1885-93
Molecular and cellular biology 2011 May;31(9):1885-93
Protective effects of the citrus flavanones to PC12 cells against cytotoxicity induced by hydrogen peroxide.
Lu YH, Su MY, Huang HY, Lin-Li, Yuan CG
Neuroscience letters 2010 Oct 22;484(1):6-11
Neuroscience letters 2010 Oct 22;484(1):6-11
Cyclosporine stimulates the renal epithelial sodium channel by elevating cholesterol.
Wang J, Zhang ZR, Chou CF, Liang YY, Gu Y, Ma HP
American journal of physiology. Renal physiology 2009 Feb;296(2):F284-90
American journal of physiology. Renal physiology 2009 Feb;296(2):F284-90
Overexpression of ABCA1 reduces amyloid deposition in the PDAPP mouse model of Alzheimer disease.
Wahrle SE, Jiang H, Parsadanian M, Kim J, Li A, Knoten A, Jain S, Hirsch-Reinshagen V, Wellington CL, Bales KR, Paul SM, Holtzman DM
The Journal of clinical investigation 2008 Feb;118(2):671-82
The Journal of clinical investigation 2008 Feb;118(2):671-82
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ABCA1 Antibody (HJ1) [NB100-2068] - Detection of ABCA1 in wildtype liver tissue (in RIPA) Lane 1: KO mouse liver tissue. Lane 2: WT mouse liver tissue.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ABCA1 Antibody (HJ1) [NB100-2068] - Analysis of ABCA1 in human renal cancer using DAB with hematoxylin counterstain.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: ABCA1 Antibody (HJ1) [NB100-2068] - Staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ABCA1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: ABCA1 Antibody (HJ1) [NB100-2068] - An intracellular stain was performed on HepG2 cells with ABCA1 Antibody (HJ1) NB100-2068PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerthrin.
