16675-1-AP

antibody from Proteintech Group

Targeting: AMFR gp78, RNF45

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16675-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16675-1-AP, RRID:AB_2226463

Product name

AMFR antibody

Antibody type

Polyclonal

Description

KD/KO validated AMFR antibody (Cat. #16675-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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Zhang Y, Wang X, Chen G, Lu Y, Chen Q
Neurological research 2024 Jan;46(1):89-97

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New mode of action of curcumin on prostate cancer cells: Modulation of endoplasmic reticulum-associated degradation mechanism and estrogenic signaling.

Erzurumlu Y, Dogan HK, Catakli D
Journal of biochemical and molecular toxicology 2024 Jan;38(1):e23636

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Standigm ASK™: knowledge graph and artificial intelligence platform applied to target discovery in idiopathic pulmonary fibrosis.

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Briefings in bioinformatics 2024 Jan 22;25(2)

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Nicotine-derived NNK promotes CRC progression through activating TMUB1/AKT pathway in METTL14/YTHDF2-mediated m6A manner.

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Journal of hazardous materials 2024 Apr 5;467:133692

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Circadian Oscillation Pattern of Endoplasmic Reticulum Quality Control (ERQC) Components in Human Embryonic Kidney HEK293 Cells.

Erzurumlu Y, Catakli D, Dogan HK
Journal of circadian rhythms 2023;21:1

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Estrogens drive the endoplasmic reticulum-associated degradation and promote proto-oncogene c-Myc expression in prostate cancer cells by androgen receptor/estrogen receptor signaling.

Erzurumlu Y, Dogan HK, Catakli D, Aydogdu E, Muhammed MT
Journal of cell communication and signaling 2023 Sep;17(3):793-811

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Triiodothyronine positively regulates endoplasmic reticulum-associated degradation (ERAD) and promotes androgenic signaling in androgen-dependent prostate cancer cells.

Erzurumlu Y, Muhammed MT
Cellular signalling 2023 Sep;109:110745

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Suberoylanilide hydroxamic acid upregulates reticulophagy receptor expression and promotes cell death in hepatocellular carcinoma cells.

Li JY, Tian T, Han B, Yang T, Guo YX, Wu JY, Chen YS, Yang Q, Xie RJ
World journal of gastroenterology 2023 Sep 14;29(34):5038-5053

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1,25(OH)(2) D(3) induced vitamin D receptor signaling negatively regulates endoplasmic reticulum-associated degradation (ERAD) and androgen receptor signaling in human prostate cancer cells.

Erzurumlu Y, Aydogdu E, Dogan HK, Catakli D, Muhammed MT, Buyuksandic B
Cellular signalling 2023 Mar;103:110577

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Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy.

Foronda H, Fu Y, Covarrubias-Pinto A, Bocker HT, González A, Seemann E, Franzka P, Bock A, Bhaskara RM, Liebmann L, Hoffmann ME, Katona I, Koch N, Weis J, Kurth I, Gleeson JG, Reggiori F, Hummer G, Kessels MM, Qualmann B, Mari M, Dikić I, Hübner CA
Nature 2023 Jun;618(7964):402-410

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Ubiquitination regulates ER-phagy and remodelling of endoplasmic reticulum.

González A, Covarrubias-Pinto A, Bhaskara RM, Glogger M, Kuncha SK, Xavier A, Seemann E, Misra M, Hoffmann ME, Bräuning B, Balakrishnan A, Qualmann B, Dötsch V, Schulman BA, Kessels MM, Hübner CA, Heilemann M, Hummer G, Dikić I
Nature 2023 Jun;618(7964):394-401

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Dexamethasone-stimulated glucocorticoid receptor signaling positively regulates the endoplasmic reticulum-associated degradation (ERAD) mechanism in hepatocellular carcinoma cells.

Erzurumlu Y, Dogan HK, Cataklı D
Steroids 2023 Jul;195:109238

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The chemokine CCL1 facilitates pulmonary fibrosis by promoting macrophage migration and M2 polarization.

Liu S, Zhang Z, Wang Y, Zhang Y, Min J, Li X, Liu S
International immunopharmacology 2023 Jul;120:110343

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Progesterone regulates the endoplasmic reticulum-associated degradation and Unfolded Protein Response axis by mimicking the androgenic stimulation in prostate cancer cells.

Erzurumlu Y, Dogan HK, Catakli D
Molecular biology reports 2023 Feb;50(2):1253-1265

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The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition.

Ganji R, Paulo JA, Xi Y, Kline I, Zhu J, Clemen CS, Weihl CC, Purdy JG, Gygi SP, Raman M
Nature communications 2023 Feb 6;14(1):638

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Gp78 deficiency in hepatocytes alleviates hepatic ischemia-reperfusion injury via suppressing ACSL4-mediated ferroptosis.

Li C, Wu Y, Chen K, Chen R, Xu S, Yang B, Lian Z, Wang X, Wang K, Xie H, Zheng S, Liu Z, Wang D, Xu X
Cell death & disease 2023 Dec 8;14(12):810

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The GR-gp78 Pathway is involved in Hepatic Lipid Accumulation Induced by Overexpression of 11β-HSD1.

Hu M, Han T, Pan Q, Ni D, Gao F, Wang L, Ren H, Zhang X, Jiao H, Wang Y, Dai D, Man Y, Tang W, Sun Y, Li W, Li J, Li G
International journal of biological sciences 2022;18(8):3107-3121

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Regulation of membrane fluidity by RNF145-triggered degradation of the lipid hydrolase ADIPOR2.

Volkmar N, Gawden-Bone CM, Williamson JC, Nixon-Abell J, West JA, St George-Hyslop PH, Kaser A, Lehner PJ
The EMBO journal 2022 Oct 4;41(19):e110777

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Basal Gp78-dependent mitophagy promotes mitochondrial health and limits mitochondrial ROS.

Alan P, Vandevoorde KR, Joshi B, Cardoen B, Gao G, Mohammadzadeh Y, Hamarneh G, Nabi IR
Cellular and molecular life sciences : CMLS 2022 Oct 25;79(11):565

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AAA-ATPase valosin-containing protein binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4.

Shibuya K, Ebihara K, Ebihara C, Sawayama N, Isoda M, Yamamuro D, Takahashi M, Nagashima S, Ishibashi S
The Journal of biological chemistry 2022 Jun;298(6):101936

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Pharmacological induction of AMFR increases functional EAAT2 oligomer levels and reduces epileptic seizures in mice.

Sha L, Li G, Zhang X, Lin Y, Qiu Y, Deng Y, Zhu W, Xu Q
JCI insight 2022 Aug 8;7(15)

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ERAD components Derlin-1 and Derlin-2 are essential for postnatal brain development and motor function.

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The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1.

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eLife 2018 Dec 13;7

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iRhom2 is essential for innate immunity to RNA virus by antagonizing ER- and mitochondria-associated degradation of VISA.

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Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.

Wang Y, Gao P, Wei C, Li H, Zhang L, Zhao Y, Wu B, Tian Y, Zhang W, Wu L, Wang R, Xu C
Cell death & disease 2017 May 18;8(5):e2799

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Aberrant corticosteroid metabolism in tumor cells enables GR takeover in enzalutamide resistant prostate cancer.

Li J, Alyamani M, Zhang A, Chang KH, Berk M, Li Z, Zhu Z, Petro M, Magi-Galluzzi C, Taplin ME, Garcia JA, Courtney K, Klein EA, Sharifi N
eLife 2017 Feb 13;6

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BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.

Llambi F, Wang YM, Victor B, Yang M, Schneider DM, Gingras S, Parsons MJ, Zheng JH, Brown SA, Pelletier S, Moldoveanu T, Chen T, Green DR
Cell 2016 Apr 7;165(2):421-33

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The E3 ubiquitin ligase AMFR and INSIG1 bridge the activation of TBK1 kinase by modifying the adaptor STING.

Wang Q, Liu X, Cui Y, Tang Y, Chen W, Li S, Yu H, Pan Y, Wang C
Immunity 2014 Dec 18;41(6):919-33

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 16675-1-AP(AMFR antibody) at dilution of 1:800

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using AMFR antibody 16675-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human ovary using 16675-1-AP(AMFR antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human ovary using 16675-1-AP(AMFR antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue