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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 16675-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#16675-1-AP, RRID:AB_2226463
- Product name
- AMFR antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated AMFR antibody (Cat. #16675-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references ERAD components Derlin-1 and Derlin-2 are essential for postnatal brain development and motor function.
Spermine Protects Cardiomyocytes from High Glucose-Induced Energy Disturbance by Targeting the CaSR-gp78-Ubiquitin Proteasome System.
Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules.
The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1.
iRhom2 is essential for innate immunity to RNA virus by antagonizing ER- and mitochondria-associated degradation of VISA.
Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.
Aberrant corticosteroid metabolism in tumor cells enables GR takeover in enzalutamide resistant prostate cancer.
BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.
The E3 ubiquitin ligase AMFR and INSIG1 bridge the activation of TBK1 kinase by modifying the adaptor STING.
Sugiyama T, Murao N, Kadowaki H, Takao K, Miyakawa T, Matsushita Y, Katagiri T, Futatsugi A, Shinmyo Y, Kawasaki H, Sakai J, Shiomi K, Nakazato M, Takeda K, Mikoshiba K, Ploegh HL, Ichijo H, Nishitoh H
iScience 2021 Jul 23;24(7):102758
iScience 2021 Jul 23;24(7):102758
Spermine Protects Cardiomyocytes from High Glucose-Induced Energy Disturbance by Targeting the CaSR-gp78-Ubiquitin Proteasome System.
Wang Y, Wang Y, Li F, Zhang X, Li H, Yang G, Xu C, Wei C
Cardiovascular drugs and therapy 2021 Feb;35(1):73-85
Cardiovascular drugs and therapy 2021 Feb;35(1):73-85
Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules.
Gao G, Zhu C, Liu E, Nabi IR
PLoS biology 2019 Aug;17(8):e3000355
PLoS biology 2019 Aug;17(8):e3000355
The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1.
Menzies SA, Volkmar N, van den Boomen DJ, Timms RT, Dickson AS, Nathan JA, Lehner PJ
eLife 2018 Dec 13;7
eLife 2018 Dec 13;7
iRhom2 is essential for innate immunity to RNA virus by antagonizing ER- and mitochondria-associated degradation of VISA.
Luo WW, Li S, Li C, Zheng ZQ, Cao P, Tong Z, Lian H, Wang SY, Shu HB, Wang YY
PLoS pathogens 2017 Nov;13(11):e1006693
PLoS pathogens 2017 Nov;13(11):e1006693
Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.
Wang Y, Gao P, Wei C, Li H, Zhang L, Zhao Y, Wu B, Tian Y, Zhang W, Wu L, Wang R, Xu C
Cell death & disease 2017 May 18;8(5):e2799
Cell death & disease 2017 May 18;8(5):e2799
Aberrant corticosteroid metabolism in tumor cells enables GR takeover in enzalutamide resistant prostate cancer.
Li J, Alyamani M, Zhang A, Chang KH, Berk M, Li Z, Zhu Z, Petro M, Magi-Galluzzi C, Taplin ME, Garcia JA, Courtney K, Klein EA, Sharifi N
eLife 2017 Feb 13;6
eLife 2017 Feb 13;6
BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.
Llambi F, Wang YM, Victor B, Yang M, Schneider DM, Gingras S, Parsons MJ, Zheng JH, Brown SA, Pelletier S, Moldoveanu T, Chen T, Green DR
Cell 2016 Apr 7;165(2):421-33
Cell 2016 Apr 7;165(2):421-33
The E3 ubiquitin ligase AMFR and INSIG1 bridge the activation of TBK1 kinase by modifying the adaptor STING.
Wang Q, Liu X, Cui Y, Tang Y, Chen W, Li S, Yu H, Pan Y, Wang C
Immunity 2014 Dec 18;41(6):919-33
Immunity 2014 Dec 18;41(6):919-33
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16675-1-AP(AMFR antibody) at dilution of 1:800
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using AMFR antibody 16675-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human ovary using 16675-1-AP(AMFR antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human ovary using 16675-1-AP(AMFR antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
