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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
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- Product number
- PA1-16535 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- XRCC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is specific for XRCC3; no cross-reactivity was observed with XRCC2, RAD51, RAD51B, RAD51C, or RAD51D. This antibody is not recommended for ICC/IF applications. Suggested positive control: antigen standard for XRCC3 (transient overexpression lysate), TK6 lysates and 100 µg HeLa lysates.
- Reactivity
- Human, Drosophila, Hamster
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references A cell-penetrating antibody inhibits human RAD51 via direct binding.
Turchick A, Hegan DC, Jensen RB, Glazer PM
Nucleic acids research 2017 Nov 16;45(20):11782-11799
Nucleic acids research 2017 Nov 16;45(20):11782-11799
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of XRCC3 (38 kDa) from Hela cell extract using Product # PA1-16535 (1:2500). Lanes 1 and 2 contain HTC116 and Hela whole cell extracts, respectively.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of XRCC3 in HTC116 and HeLa cell extract. Samples were incubated in XRCC3 polyclonal antibody (Product # PA1-16535 using a dilution of 1:2500. Lanes 1 and 2 contain HTC116 and Hela whole cell extracts, respectively.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of XRCC3 in HTC116 and HeLa cell extract. Samples were incubated in XRCC3 polyclonal antibody (Product # PA1-16535 using a dilution of 1:2500. Lanes 1 and 2 contain HTC116 and Hela whole cell extracts, respectively.
