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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- 701178 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD49f Recombinant Rabbit Monoclonal Antibody (5H14L18)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Canine
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 5H14L18
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Integrin alpha 6 in mouse lung lysate using an Integrin alpha 6 recombinant rabbit monoclonal antibody (Product # 701178) at a dilution of 1 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~119kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Integrin alpha 6 in mouse lung lysate using an Integrin alpha 6 recombinant rabbit monoclonal antibody (Product # 701178) at a dilution of 1 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~119kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane1), MDCK (Lane2), CaCo-2 (Lane 3) and A549 (Lane4). The blots were probed with Integrin alpha 6 Rabbit Monoclonal Antibody (Product # 701178, 2-3 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 150 kDa band corresponding to Integrin alpha 6 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with Pierce™ Power Blotter System (22834) The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
