Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA324731 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal ITGA6 Antibody (isoform 2 S1064)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal ITGA6 Antibody (isoform 2 S1064)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ITGA6
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.33 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-ITGA6 Antibody (isoform 2 S1064) (Cat.#TA324731) in 293 cell line lysates (35ug/lane). ITGA6(arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of HeLa cells stained with ITGA6 (isoform 2 S1064) antibody. HeLa cells were incubated with TA324731 ITGA6 (isoform 2 S1064) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . Note the highly specific localization of the ITGA6 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000091409).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with ITGA6 Antibody (isoform 2 S1064) (Cat.#TA324731), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC