Antibody data
- Antibody Data
- Antigen structure
- References [25]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-0495-81 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex; CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The eBioGoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin. Applications Reported: This eBioGoH3 (GoH3) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. Applications Tested: This eBioGoH3 (GoH3) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- eBioGoH3 (GoH3)
- Vial size
- 50 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Integrin-Rac signalling for mammary epithelial stem cell self-renewal.
Inflammatory memory sensitizes skin epithelial stem cells to tissue damage.
Low Testosterone Alters the Activity of Mouse Prostate Stem Cells.
Llgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration.
Yap1 promotes the survival and self-renewal of breast tumor initiating cells via inhibiting Smad3 signaling.
Stem cell function and stress response are controlled by protein synthesis.
Characterization of the CD49f+/CD44+/CD24- single-cell derived stem cell population in basal-like DCIS cells.
Inhibition of the glucocorticoid receptor results in an enhanced miR-99a/100-mediated radiation response in stem-like cells from human prostate cancers.
Directed elimination of senescent cells by inhibition of BCL-W and BCL-XL.
Enhanced Expression of Integrin αvβ3 Induced by TGF-β Is Required for the Enhancing Effect of Fibroblast Growth Factor 1 (FGF1) in TGF-β-Induced Epithelial-Mesenchymal Transition (EMT) in Mammary Epithelial Cells.
Merlin Isoforms 1 and 2 Both Act as Tumour Suppressors and Are Required for Optimal Sperm Maturation.
The Transcriptional Repressor ZNF503/Zeppo2 Promotes Mammary Epithelial Cell Proliferation and Enhances Cell Invasion.
FGF ligands of the postnatal mammary stroma regulate distinct aspects of epithelial morphogenesis.
Force engages vinculin and promotes tumor progression by enhancing PI3K activation of phosphatidylinositol (3,4,5)-triphosphate.
Highly upregulated Lhx2 in the Foxn1-/- nude mouse phenotype reflects a dysregulated and expanded epidermal stem cell niche.
The CD9/CD81 tetraspanin complex and tetraspanin CD151 regulate α3β1 integrin-dependent tumor cell behaviors by overlapping but distinct mechanisms.
Distinct effects of EGFR ligands on human mammary epithelial cell differentiation.
Evidence for a multipotent mammary progenitor with pregnancy-specific activity.
Generation of a novel germline stem cell line expressing a germline-specific reporter in the mouse.
GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.
P2Y2 receptor inhibits EGF-induced MAPK pathway to stabilise keratinocyte hemidesmosomes.
The interplay between surfaces and soluble factors define the immunologic and angiogenic properties of myeloid dendritic cells.
Hepatoblast-like progenitor cells derived from embryonic stem cells can repopulate livers of mice.
Antibody to integrin alpha 6 subunit specifically inhibits cell-binding to laminin fragment 8.
Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin.
Olabi S, Ucar A, Brennan K, Streuli CH
Breast cancer research : BCR 2018 Oct 22;20(1):128
Breast cancer research : BCR 2018 Oct 22;20(1):128
Inflammatory memory sensitizes skin epithelial stem cells to tissue damage.
Naik S, Larsen SB, Gomez NC, Alaverdyan K, Sendoel A, Yuan S, Polak L, Kulukian A, Chai S, Fuchs E
Nature 2017 Oct 26;550(7677):475-480
Nature 2017 Oct 26;550(7677):475-480
Low Testosterone Alters the Activity of Mouse Prostate Stem Cells.
Zhou Y, Copeland B, Otto-Duessel M, He M, Markel S, Synold TW, Jones JO
The Prostate 2017 Apr;77(5):530-541
The Prostate 2017 Apr;77(5):530-541
Llgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration.
Greenwood E, Maisel S, Ebertz D, Russ A, Pandey R, Schroeder J
Oncotarget 2016 Sep 20;7(38):60776-60792
Oncotarget 2016 Sep 20;7(38):60776-60792
Yap1 promotes the survival and self-renewal of breast tumor initiating cells via inhibiting Smad3 signaling.
Sun JG, Chen XW, Zhang LP, Wang J, Diehn M
Oncotarget 2016 Mar 1;7(9):9692-706
Oncotarget 2016 Mar 1;7(9):9692-706
Stem cell function and stress response are controlled by protein synthesis.
Blanco S, Bandiera R, Popis M, Hussain S, Lombard P, Aleksic J, Sajini A, Tanna H, Cortés-Garrido R, Gkatza N, Dietmann S, Frye M
Nature 2016 Jun 16;534(7607):335-40
Nature 2016 Jun 16;534(7607):335-40
Characterization of the CD49f+/CD44+/CD24- single-cell derived stem cell population in basal-like DCIS cells.
Duru N, Gernapudi R, Lo PK, Yao Y, Wolfson B, Zhang Y, Zhou Q
Oncotarget 2016 Jul 26;7(30):47511-47525
Oncotarget 2016 Jul 26;7(30):47511-47525
Inhibition of the glucocorticoid receptor results in an enhanced miR-99a/100-mediated radiation response in stem-like cells from human prostate cancers.
Rane JK, Erb HH, Nappo G, Mann VM, Simms MS, Collins AT, Visakorpi T, Maitland NJ
Oncotarget 2016 Aug 9;7(32):51965-51980
Oncotarget 2016 Aug 9;7(32):51965-51980
Directed elimination of senescent cells by inhibition of BCL-W and BCL-XL.
Yosef R, Pilpel N, Tokarsky-Amiel R, Biran A, Ovadya Y, Cohen S, Vadai E, Dassa L, Shahar E, Condiotti R, Ben-Porath I, Krizhanovsky V
Nature communications 2016 Apr 6;7:11190
Nature communications 2016 Apr 6;7:11190
Enhanced Expression of Integrin αvβ3 Induced by TGF-β Is Required for the Enhancing Effect of Fibroblast Growth Factor 1 (FGF1) in TGF-β-Induced Epithelial-Mesenchymal Transition (EMT) in Mammary Epithelial Cells.
Mori S, Kodaira M, Ito A, Okazaki M, Kawaguchi N, Hamada Y, Takada Y, Matsuura N
PloS one 2015;10(9):e0137486
PloS one 2015;10(9):e0137486
Merlin Isoforms 1 and 2 Both Act as Tumour Suppressors and Are Required for Optimal Sperm Maturation.
Zoch A, Mayerl S, Schulz A, Greither T, Frappart L, Rübsam J, Heuer H, Giovannini M, Morrison H
PloS one 2015;10(8):e0129151
PloS one 2015;10(8):e0129151
The Transcriptional Repressor ZNF503/Zeppo2 Promotes Mammary Epithelial Cell Proliferation and Enhances Cell Invasion.
Shahi P, Slorach EM, Wang CY, Chou J, Lu A, Ruderisch A, Werb Z
The Journal of biological chemistry 2015 Feb 6;290(6):3803-13
The Journal of biological chemistry 2015 Feb 6;290(6):3803-13
FGF ligands of the postnatal mammary stroma regulate distinct aspects of epithelial morphogenesis.
Zhang X, Martinez D, Koledova Z, Qiao G, Streuli CH, Lu P
Development (Cambridge, England) 2014 Sep;141(17):3352-62
Development (Cambridge, England) 2014 Sep;141(17):3352-62
Force engages vinculin and promotes tumor progression by enhancing PI3K activation of phosphatidylinositol (3,4,5)-triphosphate.
Rubashkin MG, Cassereau L, Bainer R, DuFort CC, Yui Y, Ou G, Paszek MJ, Davidson MW, Chen YY, Weaver VM
Cancer research 2014 Sep 1;74(17):4597-611
Cancer research 2014 Sep 1;74(17):4597-611
Highly upregulated Lhx2 in the Foxn1-/- nude mouse phenotype reflects a dysregulated and expanded epidermal stem cell niche.
Bohr S, Patel SJ, Vasko R, Shen K, Huang G, Yarmush ML, Berthiaume F
PloS one 2013;8(5):e64223
PloS one 2013;8(5):e64223
The CD9/CD81 tetraspanin complex and tetraspanin CD151 regulate α3β1 integrin-dependent tumor cell behaviors by overlapping but distinct mechanisms.
Gustafson-Wagner E, Stipp CS
PloS one 2013;8(4):e61834
PloS one 2013;8(4):e61834
Distinct effects of EGFR ligands on human mammary epithelial cell differentiation.
Mukhopadhyay C, Zhao X, Maroni D, Band V, Naramura M
PloS one 2013;8(10):e75907
PloS one 2013;8(10):e75907
Evidence for a multipotent mammary progenitor with pregnancy-specific activity.
Kaanta AS, Virtanen C, Selfors LM, Brugge JS, Neel BG
Breast cancer research : BCR 2013;15(4):R65
Breast cancer research : BCR 2013;15(4):R65
Generation of a novel germline stem cell line expressing a germline-specific reporter in the mouse.
Shiura H, Ikeda R, Lee J, Sato T, Ogonuki N, Hirose M, Ogura A, Ogawa T, Abe K
Genesis (New York, N.Y. : 2000) 2013 Jul;51(7):498-505
Genesis (New York, N.Y. : 2000) 2013 Jul;51(7):498-505
GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.
Chou J, Lin JH, Brenot A, Kim JW, Provot S, Werb Z
Nature cell biology 2013 Feb;15(2):201-13
Nature cell biology 2013 Feb;15(2):201-13
P2Y2 receptor inhibits EGF-induced MAPK pathway to stabilise keratinocyte hemidesmosomes.
Faure E, Garrouste F, Parat F, Monferran S, Leloup L, Pommier G, Kovacic H, Lehmann M
Journal of cell science 2012 Sep 15;125(Pt 18):4264-77
Journal of cell science 2012 Sep 15;125(Pt 18):4264-77
The interplay between surfaces and soluble factors define the immunologic and angiogenic properties of myeloid dendritic cells.
Sprague L, Muccioli M, Pate M, Meles E, McGinty J, Nandigam H, Venkatesh AK, Gu MY, Mansfield K, Rutowski A, Omosebi O, Courreges MC, Benencia F
BMC immunology 2011 Jun 6;12:35
BMC immunology 2011 Jun 6;12:35
Hepatoblast-like progenitor cells derived from embryonic stem cells can repopulate livers of mice.
Li F, Liu P, Liu C, Xiang D, Deng L, Li W, Wangensteen K, Song J, Ma Y, Hui L, Wei L, Li L, Ding X, Hu Y, He Z, Wang X
Gastroenterology 2010 Dec;139(6):2158-2169.e8
Gastroenterology 2010 Dec;139(6):2158-2169.e8
Antibody to integrin alpha 6 subunit specifically inhibits cell-binding to laminin fragment 8.
Aumailley M, Timpl R, Sonnenberg A
Experimental cell research 1990 May;188(1):55-60
Experimental cell research 1990 May;188(1):55-60
Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin.
Sonnenberg A, Daams H, Van der Valk MA, Hilkens J, Hilgers J
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1986 Aug;34(8):1037-46
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1986 Aug;34(8):1037-46
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.25 µg of Rat IgG2a Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.25 µg of Anti-Human/Mouse CD49f (Integrin alpha 6) Purified (filled histogram) followed by Anti-Rat IgG PE (Product # 12-4822). Cells in the lymphocyte gate were used for analysis.