Antibody data
- Antibody Data
- Antigen structure
- References [23]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 46-0495-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), PerCP-eFluor 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex; CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The eBioGoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin. Applications Reported: This eBioGoH3 (GoH3) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioGoH3 (GoH3) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- eBioGoH3 (GoH3)
- Vial size
- 25 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Integrin-Rac signalling for mammary epithelial stem cell self-renewal.
Inflammatory memory sensitizes skin epithelial stem cells to tissue damage.
KDM4 Inhibition Targets Breast Cancer Stem-like Cells.
Low Testosterone Alters the Activity of Mouse Prostate Stem Cells.
Llgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration.
Yap1 promotes the survival and self-renewal of breast tumor initiating cells via inhibiting Smad3 signaling.
Characterization of the CD49f+/CD44+/CD24- single-cell derived stem cell population in basal-like DCIS cells.
Inhibition of the glucocorticoid receptor results in an enhanced miR-99a/100-mediated radiation response in stem-like cells from human prostate cancers.
Directed elimination of senescent cells by inhibition of BCL-W and BCL-XL.
Merlin Isoforms 1 and 2 Both Act as Tumour Suppressors and Are Required for Optimal Sperm Maturation.
Foxp1 Regulates the Proliferation of Hair Follicle Stem Cells in Response to Oxidative Stress during Hair Cycling.
Development of a diverse human T-cell repertoire despite stringent restriction of hematopoietic clonality in the thymus.
The Transcriptional Repressor ZNF503/Zeppo2 Promotes Mammary Epithelial Cell Proliferation and Enhances Cell Invasion.
FGF ligands of the postnatal mammary stroma regulate distinct aspects of epithelial morphogenesis.
Sustained Engraftment of Cryopreserved Human Bone Marrow CD34(+) Cells in Young Adult NSG Mice.
Highly upregulated Lhx2 in the Foxn1-/- nude mouse phenotype reflects a dysregulated and expanded epidermal stem cell niche.
Distinct effects of EGFR ligands on human mammary epithelial cell differentiation.
Evidence for a multipotent mammary progenitor with pregnancy-specific activity.
Generation of a novel germline stem cell line expressing a germline-specific reporter in the mouse.
GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.
Alpha6-integrin is expressed on germinal centre B cells and modifies growth of a B-cell line.
Antibody to integrin alpha 6 subunit specifically inhibits cell-binding to laminin fragment 8.
Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin.
Olabi S, Ucar A, Brennan K, Streuli CH
Breast cancer research : BCR 2018 Oct 22;20(1):128
Breast cancer research : BCR 2018 Oct 22;20(1):128
Inflammatory memory sensitizes skin epithelial stem cells to tissue damage.
Naik S, Larsen SB, Gomez NC, Alaverdyan K, Sendoel A, Yuan S, Polak L, Kulukian A, Chai S, Fuchs E
Nature 2017 Oct 26;550(7677):475-480
Nature 2017 Oct 26;550(7677):475-480
KDM4 Inhibition Targets Breast Cancer Stem-like Cells.
Metzger E, Stepputtis SS, Strietz J, Preca BT, Urban S, Willmann D, Allen A, Zenk F, Iovino N, Bronsert P, Proske A, Follo M, Boerries M, Stickeler E, Xu J, Wallace MB, Stafford JA, Kanouni T, Maurer J, Schüle R
Cancer research 2017 Nov 1;77(21):5900-5912
Cancer research 2017 Nov 1;77(21):5900-5912
Low Testosterone Alters the Activity of Mouse Prostate Stem Cells.
Zhou Y, Copeland B, Otto-Duessel M, He M, Markel S, Synold TW, Jones JO
The Prostate 2017 Apr;77(5):530-541
The Prostate 2017 Apr;77(5):530-541
Llgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration.
Greenwood E, Maisel S, Ebertz D, Russ A, Pandey R, Schroeder J
Oncotarget 2016 Sep 20;7(38):60776-60792
Oncotarget 2016 Sep 20;7(38):60776-60792
Yap1 promotes the survival and self-renewal of breast tumor initiating cells via inhibiting Smad3 signaling.
Sun JG, Chen XW, Zhang LP, Wang J, Diehn M
Oncotarget 2016 Mar 1;7(9):9692-706
Oncotarget 2016 Mar 1;7(9):9692-706
Characterization of the CD49f+/CD44+/CD24- single-cell derived stem cell population in basal-like DCIS cells.
Duru N, Gernapudi R, Lo PK, Yao Y, Wolfson B, Zhang Y, Zhou Q
Oncotarget 2016 Jul 26;7(30):47511-47525
Oncotarget 2016 Jul 26;7(30):47511-47525
Inhibition of the glucocorticoid receptor results in an enhanced miR-99a/100-mediated radiation response in stem-like cells from human prostate cancers.
Rane JK, Erb HH, Nappo G, Mann VM, Simms MS, Collins AT, Visakorpi T, Maitland NJ
Oncotarget 2016 Aug 9;7(32):51965-51980
Oncotarget 2016 Aug 9;7(32):51965-51980
Directed elimination of senescent cells by inhibition of BCL-W and BCL-XL.
Yosef R, Pilpel N, Tokarsky-Amiel R, Biran A, Ovadya Y, Cohen S, Vadai E, Dassa L, Shahar E, Condiotti R, Ben-Porath I, Krizhanovsky V
Nature communications 2016 Apr 6;7:11190
Nature communications 2016 Apr 6;7:11190
Merlin Isoforms 1 and 2 Both Act as Tumour Suppressors and Are Required for Optimal Sperm Maturation.
Zoch A, Mayerl S, Schulz A, Greither T, Frappart L, Rübsam J, Heuer H, Giovannini M, Morrison H
PloS one 2015;10(8):e0129151
PloS one 2015;10(8):e0129151
Foxp1 Regulates the Proliferation of Hair Follicle Stem Cells in Response to Oxidative Stress during Hair Cycling.
Zhao J, Li H, Zhou R, Ma G, Dekker JD, Tucker HO, Yao Z, Guo X
PloS one 2015;10(7):e0131674
PloS one 2015;10(7):e0131674
Development of a diverse human T-cell repertoire despite stringent restriction of hematopoietic clonality in the thymus.
Brugman MH, Wiekmeijer AS, van Eggermond M, Wolvers-Tettero I, Langerak AW, de Haas EF, Bystrykh LV, van Rood JJ, de Haan G, Fibbe WE, Staal FJ
Proceedings of the National Academy of Sciences of the United States of America 2015 Nov 3;112(44):E6020-7
Proceedings of the National Academy of Sciences of the United States of America 2015 Nov 3;112(44):E6020-7
The Transcriptional Repressor ZNF503/Zeppo2 Promotes Mammary Epithelial Cell Proliferation and Enhances Cell Invasion.
Shahi P, Slorach EM, Wang CY, Chou J, Lu A, Ruderisch A, Werb Z
The Journal of biological chemistry 2015 Feb 6;290(6):3803-13
The Journal of biological chemistry 2015 Feb 6;290(6):3803-13
FGF ligands of the postnatal mammary stroma regulate distinct aspects of epithelial morphogenesis.
Zhang X, Martinez D, Koledova Z, Qiao G, Streuli CH, Lu P
Development (Cambridge, England) 2014 Sep;141(17):3352-62
Development (Cambridge, England) 2014 Sep;141(17):3352-62
Sustained Engraftment of Cryopreserved Human Bone Marrow CD34(+) Cells in Young Adult NSG Mice.
Wiekmeijer AS, Pike-Overzet K, Brugman MH, Salvatori DC, Egeler RM, Bredius RG, Fibbe WE, Staal FJ
BioResearch open access 2014 Jun 1;3(3):110-6
BioResearch open access 2014 Jun 1;3(3):110-6
Highly upregulated Lhx2 in the Foxn1-/- nude mouse phenotype reflects a dysregulated and expanded epidermal stem cell niche.
Bohr S, Patel SJ, Vasko R, Shen K, Huang G, Yarmush ML, Berthiaume F
PloS one 2013;8(5):e64223
PloS one 2013;8(5):e64223
Distinct effects of EGFR ligands on human mammary epithelial cell differentiation.
Mukhopadhyay C, Zhao X, Maroni D, Band V, Naramura M
PloS one 2013;8(10):e75907
PloS one 2013;8(10):e75907
Evidence for a multipotent mammary progenitor with pregnancy-specific activity.
Kaanta AS, Virtanen C, Selfors LM, Brugge JS, Neel BG
Breast cancer research : BCR 2013;15(4):R65
Breast cancer research : BCR 2013;15(4):R65
Generation of a novel germline stem cell line expressing a germline-specific reporter in the mouse.
Shiura H, Ikeda R, Lee J, Sato T, Ogonuki N, Hirose M, Ogura A, Ogawa T, Abe K
Genesis (New York, N.Y. : 2000) 2013 Jul;51(7):498-505
Genesis (New York, N.Y. : 2000) 2013 Jul;51(7):498-505
GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.
Chou J, Lin JH, Brenot A, Kim JW, Provot S, Werb Z
Nature cell biology 2013 Feb;15(2):201-13
Nature cell biology 2013 Feb;15(2):201-13
Alpha6-integrin is expressed on germinal centre B cells and modifies growth of a B-cell line.
Ambrose HE, Wagner SD
Immunology 2004 Apr;111(4):400-6
Immunology 2004 Apr;111(4):400-6
Antibody to integrin alpha 6 subunit specifically inhibits cell-binding to laminin fragment 8.
Aumailley M, Timpl R, Sonnenberg A
Experimental cell research 1990 May;188(1):55-60
Experimental cell research 1990 May;188(1):55-60
Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin.
Sonnenberg A, Daams H, Van der Valk MA, Hilkens J, Hilgers J
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1986 Aug;34(8):1037-46
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1986 Aug;34(8):1037-46
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.03 µg of Rat IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (blue histogram) or 0.03 µg of Anti-Human/Mouse CD49f (Integrin alpha 6) PerCP-eFluor® 710 (purple histogram). Cells in the lymphocyte gate were used for analysis.