Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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Validation data
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- Product number
- PA5-21324 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD49f Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Human ESC, Rat lung. Predicted reactivity: Mouse (94%), Rat (93%), Rhesus Monkey (99%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.64 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Integrin alpha 6 in Rat tissue extract (50 µg). Samples was separated by 7.5% SDS-PAGE and the membrane was probed with Integrin alpha 6, Internal Polyclonal antibody (Product # PA5-21324) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CD49f Polyclonal Antibody (Product # PA5-21324). Sample (20 µg of whole cell lysate). Lane A: human ESC. 5% SDS PAGE. CD49f Polyclonal Antibody (Product # PA5-21324) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CD49f Polyclonal Antibody (Product # PA5-21324). Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD49f Polyclonal Antibody (Product # PA5-21324) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CD49f Polyclonal Antibody (Product # PA5-21324). Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD49f Polyclonal Antibody (Product # PA5-21324) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.