MA5-27769

antibody from Invitrogen Antibodies

Targeting: TONSL IKBR, NFKBIL2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27769

Provider

Invitrogen Antibodies

Product name

NFKBIL2 Monoclonal Antibody (GT648)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: 293T, A431, HeLa, HepG2 Predicted Reactivity: Mouse (83%), Rat (82%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

GT648

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Loss of MED12 activates the TGFβ pathway to promote chemoresistance and replication fork stability in BRCA-deficient cells.

Jackson LM, Dhoonmoon A, Hale A, Dennis KA, Schleicher EM, Nicolae CM, Moldovan GL
Nucleic acids research 2021 Dec 16;49(22):12855-12869

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NFKBIL2 in A) 293T whole cell lysate, B) A431 whole cell lysate, C) HeLa whole cell lysate, D) HepG2 whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with NFKBIL2 monoclonal antibody (Product # MA5-27769) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NFKBIL2 Monoclonal Antibody (GT648) (Product # MA5-27769). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NFKBIL2 Monoclonal Antibody (GT648) (Product # MA5-27769) diluted at 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.