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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- GTX105870 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105870, RRID:AB_1950902
- Product name
- MDH2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin-resistant uterine cancer.
Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
Hyaluronic acid-dependent protection against UVB-damaged human corneal cells.
Lo YW, Lin ST, Chang SJ, Chan CH, Lyu KW, Chang JF, May EW, Lin DY, Chou HC, Chan HL
Journal of cellular and molecular medicine 2015 Apr;19(4):744-59
Journal of cellular and molecular medicine 2015 Apr;19(4):744-59
Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
May EW, Lin ST, Lin CC, Chang JF, Hung E, Lo YW, Lin LH, Hu RY, Feng CL, Lin DY, Wu SB, Lee WC, Lyu KW, Chou HC, Chan HL
Pharmacological research 2014 Dec;90:1-17
Pharmacological research 2014 Dec;90:1-17
Hyaluronic acid-dependent protection against UVB-damaged human corneal cells.
Li JM, Chou HC, Wang SH, Wu CL, Chen YW, Lin ST, Chen YH, Chan HL
Environmental and molecular mutagenesis 2013 Jul;54(6):429-49
Environmental and molecular mutagenesis 2013 Jul;54(6):429-49
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample(30 ?g of whole cell lysate)A:293T 12% SDS PAGEGTX105870 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ?g of whole cell lysate) A: mouse brain 10% SDS PAGE GTX105870 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MDH2 antibody detects MDH2 protein by western blot analysis.A. 50 ?g rat lung lysate/extract10% SDS-PAGEMDH2 antibody (GTX105870) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and MDH2 knockout (KO) 293T cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody (GTX105870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody (GTX105870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MDH2 antibody detects MDH2 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: MDH2 protein stained by MDH2 antibody (GTX105870) diluted at 1:500.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MDH2 antibody detects MDH2 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: MDH2 stained by MDH2 antibody (GTX105870) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10£gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MDH2 antibody detects MDH2 protein at cytosol on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. MDH2 antibody (GTX105870) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MDH2 antibody detects MDH2 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MDH2 antibody (GTX105870) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MDH2 antibody detects MDH2 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. MDH2 antibody (GTX105870) diluted at 1:500.
