Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-118508 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ILKAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total ILKAP.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ILKAP in multiple tissue types using a ILKAP polyclonal antibody (Product # PA5-118508). Lane 1:Mouse heart lysates; Lane 2: Mouse lung lysates; Lane 3: Mouse brain lysates; Lane 4: Rat liver lysates.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ILKAP in RAW264.7 cells(LPS 4h treatment),K562 cells(heat shock treatment) using a ILKAP polyclonal antibody (Product # PA5-118508). Lane 1:RAW264.7 cells(LPS 4h treatment), blocked with antigen-specific peptides, Lane 2: RAW264.7 cells(LPS 4h treatment), Lane 3: K562 cells(heat shock treatment).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of ILKAP in A549 cells using a ILKAP polyclonal antibody (Product # PA5-118508). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with the primary antibody and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ILKAP in human Smooth muscle tissue using a ILKAP polyclonal antibody (Product # PA5-118508). The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ILKAP in Mouse muscle tissue using a ILKAP polyclonal antibody (Product # PA5-118508). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ILKAP in Rat heart tissue using a ILKAP polyclonal antibody (Product # PA5-118508). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ILKAP in Rat skin tissue using a ILKAP polyclonal antibody (Product # PA5-118508). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.