Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB839F-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-12 R beta 1 Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-12 R beta 1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-12 R beta 1 is observed.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
P42701
- Isotype
- IgG
- Antibody clone number
- 69310
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity.
Dendrou CA, Cortes A, Shipman L, Evans HG, Attfield KE, Jostins L, Barber T, Kaur G, Kuttikkatte SB, Leach OA, Desel C, Faergeman SL, Cheeseman J, Neville MJ, Sawcer S, Compston A, Johnson AR, Everett C, Bell JI, Karpe F, Ultsch M, Eigenbrot C, McVean G, Fugger L
Science translational medicine 2016 Nov 2;8(363):363ra149
Science translational medicine 2016 Nov 2;8(363):363ra149
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of IL-12 R beta 1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 5 μg/mL PHA for 48 hours (B) untreated, were stained with Mouse Anti-Human IL-12 R beta 1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB839F) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.