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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-27646 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Talin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, A549, H1299, HCT116. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (92%), Chicken (93%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TLN1 using Various whole cell extracts (30 µg). Samples were loaded onto a 5% SDS-PAGE gel and probed with a TLN1 polyclonal antibody (Product # PA5-27646) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Talin 1 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Talin 1 Polyclonal Antibody (Product # PA5-27646) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Talin 1 antibody detects Talin 1 protein by Western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Talin 1 antibody Talin 1 Polyclonal Antibody (Product # PA5-27646) diluted by 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Talin 1 antibody [C3], C-term detects TLN1 protein at cytosol on H357 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H357 xenograft. Talin 1 antibody [C3], C-term (Product # PA5-27646) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
