PA5-27646

antibody from Invitrogen Antibodies

Targeting: TLN1 ILWEQ, TLN

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27646

Provider

Invitrogen Antibodies

Product name

Talin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, A549, H1299, HCT116.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TLN1 using Various whole cell extracts (30 µg). Samples were loaded onto a 5% SDS-PAGE gel and probed with a TLN1 polyclonal antibody (Product # PA5-27646) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Talin 1 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Talin 1 Polyclonal Antibody (Product # PA5-27646) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Talin 1 antibody detects Talin 1 protein by Western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Talin 1 antibody Talin 1 Polyclonal Antibody (Product # PA5-27646) diluted by 1:500.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Talin 1 antibody [C3], C-term detects TLN1 protein at cytosol on H357 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H357 xenograft. Talin 1 antibody [C3], C-term (Product # PA5-27646) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.