Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BAF1219 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Integrin alpha V/CD51 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Integrin alpha V/CD51 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) Integrin alpha V is observed, 5% cross-reactivity with recombinant human (rh) Integrin alpha 2 is observed, and less than 1% cross-reactivity with rhIntegrin alpha 5 and rmIntegrin alpha E is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
NP_002201- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Integrin alpha V/CD51 in Human Mesenchymal Stem Cells. Integrin alpha V/CD51 was detected in immersion fixed human mesenchymal stem cells using Goat Anti-Human Integrin alpha V/CD51 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
