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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB1219 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Integrin alpha V/CD51 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects the ectodomain of human Integrin alpha V/CD51.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- P2W7
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Plasma endothelial microparticles reflect the extent of capillaroscopic alterations and correlate with the severity of skin involvement in systemic sclerosis.
Entamoeba histolytica Cysteine Proteinase 5 Evokes Mucin Exocytosis from Colonic Goblet Cells via αvβ3 Integrin.
Heparin inhibits the interaction of DNA topoisomerase I/anti-topoisomerase I immune complexes with heparan sulfate on dermal fibroblasts.
Agrin, a novel basement membrane component in human and rat liver, accumulates in cirrhosis and hepatocellular carcinoma.
Michalska-Jakubus M, Kowal-Bielecka O, Smith V, Cutolo M, Krasowska D
Microvascular research 2017 Mar;110:24-31
Microvascular research 2017 Mar;110:24-31
Entamoeba histolytica Cysteine Proteinase 5 Evokes Mucin Exocytosis from Colonic Goblet Cells via αvβ3 Integrin.
Cornick S, Moreau F, Chadee K
PLoS pathogens 2016 Apr;12(4):e1005579
PLoS pathogens 2016 Apr;12(4):e1005579
Heparin inhibits the interaction of DNA topoisomerase I/anti-topoisomerase I immune complexes with heparan sulfate on dermal fibroblasts.
Arcand J, Robitaille G, Koenig M, Senécal JL, Raymond Y
Arthritis and rheumatism 2012 May;64(5):1632-41
Arthritis and rheumatism 2012 May;64(5):1632-41
Agrin, a novel basement membrane component in human and rat liver, accumulates in cirrhosis and hepatocellular carcinoma.
Tátrai P, Dudás J, Batmunkh E, Máthé M, Zalatnai A, Schaff Z, Ramadori G, Kovalszky I
Laboratory investigation; a journal of technical methods and pathology 2006 Nov;86(11):1149-60
Laboratory investigation; a journal of technical methods and pathology 2006 Nov;86(11):1149-60
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Integrin alpha V/CD51 in HT1080 Human Cell Line. Integrin alpha V/CD51 was detected in immersion fixed HT1080 human fibrosarcoma cell line using 10 µg/mL Mouse Anti-Human Integrin alpha V/CD51 Monoclonal Antibody (Catalog # MAB1219) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Integrin alpha V/CD51 in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin alpha V/CD51 Monoclonal Antibody (Catalog # MAB1219, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B).
