MA5-17123

antibody from Invitrogen Antibodies

Targeting: MMP3 STMY, STMY1

ELISA

Antibody data

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Validation data

Product number: MA5-17123 - Provider product page
Provider: Invitrogen Antibodies
Product name: MMP3 Monoclonal Antibody (4F10)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-17123 targets MMP3 in FACS, indirect ELISA, and WB applications and shows reactivity with Human samples. The MA5-17123 immunogen is purified recombinant fragment of human MMP3 expressed in E. Coli. MA5-17123 detects MMP3 which has a predicted molecular weight of approximately 54kDa.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: 4F10
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

MMP3 protein structure - MA5-17123 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MMP3 using a MMP3 monoclonal antibody (Product # MA5-17123) against a human MMP3 (AA: 189-441) recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of MMP3 was achieved by transfecting HeLa cells with MMP3 specific siRNAs (Silencer® select Product # s8854, s8855). Western blot analysis (Fig. a) was performed using whole cell extracts from the MMP3 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed by MMP3 Monoclonal Antibody (Product # MA5-17123) and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MMP3.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-MMP3 Monoclonal Antibody (4F10), (Product # MA5-17123) and a 53.9 kDa band corresponding to MMP3 was observed in the cell lines. Whole cell extracts (30 µg lysate) of C8D1A (Lane 1), HeLa (Lane 2) and MDA-MB-231 (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of MMP3 using a MMP3 monoclonal antibody (Product # MA5-17123) against a human MMP3 (AA: 189-441) recombinant protein.