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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB4795 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4795, RRID:AB_1577364
- Product name
- MMP3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MMP3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cleavage of the matricellular protein SPARC by matrix metalloproteinase 3 produces polypeptides that influence angiogenesis.
Matrix metalloproteinases as novel disease markers in Takayasu arteritis.
Stromelysin-1/matrix metalloproteinase-3 (MMP-3) expression accounts for invasive properties of human astrocytoma cell lines.
Sage EH, Reed M, Funk SE, Truong T, Steadele M, Puolakkainen P, Maurice DH, Bassuk JA
The Journal of biological chemistry 2003 Sep 26;278(39):37849-57
The Journal of biological chemistry 2003 Sep 26;278(39):37849-57
Matrix metalloproteinases as novel disease markers in Takayasu arteritis.
Matsuyama A, Sakai N, Ishigami M, Hiraoka H, Kashine S, Hirata A, Nakamura T, Yamashita S, Matsuzawa Y
Circulation 2003 Sep 23;108(12):1469-73
Circulation 2003 Sep 23;108(12):1469-73
Stromelysin-1/matrix metalloproteinase-3 (MMP-3) expression accounts for invasive properties of human astrocytoma cell lines.
Mercapide J, Lopez De Cicco R, Castresana JS, Klein-Szanto AJ
International journal of cancer 2003 Sep 20;106(5):676-82
International journal of cancer 2003 Sep 20;106(5):676-82
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MMP3 (arrow) using rabbit MMP3 polyclonal antibody (Cat # PAB4795). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP3 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MMP3 polyclonal antibody (Cat # PAB4795) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using MMP3 polyclonal antibody (Cat # PAB4795) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
