MA5-14210

antibody from Invitrogen Antibodies

Targeting: MMP3 STMY, STMY1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14210

Provider

Invitrogen Antibodies

Product name

MMP3 Monoclonal Antibody (SL-1 IID4)

Antibody type

Monoclonal

Antigen

Other

Description

MA5-14210 targets MMP-3 (Stromelysin-1) in IF and WB applications and shows reactivity with Human samples. The MA5-14210 immunogen is aPMA (4-Aminophenylmercuric acetate) activated Human stromelysin-1 (SL-1).

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

SL-1 IID4

Vial size

500 µL

Concentration

0.2 mg/mL

Storage

4° C

References

Submitted references

Immunoexpression of matrix metalloproteinases and their inhibitors in different areas of oral squamous cell carcinoma.

Suarez-Roa ML, Asbun-Bojalil J, Ruiz-Godoy LM, Meneses-García AA
Australian dental journal 2012 Sep;57(3):300-7

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Creation, validation, and quantitative analysis of protein expression in vascular tissue microarrays.

Halushka MK, Cornish TC, Lu J, Selvin S, Selvin E
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology 2010 May-Jun;19(3):136-46

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Inflammatory infiltrates and neovessels are relevant sources of MMPs in abdominal aortic aneurysm wall.

Reeps C, Pelisek J, Seidl S, Schuster T, Zimmermann A, Kuehnl A, Eckstein HH
Pathobiology : journal of immunopathology, molecular and cellular biology 2009;76(5):243-52

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Cigarette smoke condensate affects the collagen-degrading ability of human gingival fibroblasts.

Zhang W, Song F, Windsor LJ
Journal of periodontal research 2009 Dec;44(6):704-13

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Nicotine increases the collagen-degrading ability of human gingival fibroblasts.

Zhou J, Olson BL, Windsor LJ
Journal of periodontal research 2007 Jun;42(3):228-35

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Modulation of extracellular matrix components by metalloproteinases and their tissue inhibitors during degeneration and regeneration of rat sural nerve.

Gantus MA, Nasciutti LE, Cruz CM, Persechini PM, Martinez AM
Brain research 2006 Nov 29;1122(1):36-46

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Interleukin-1 alpha alters the expression of matrix metalloproteinases and collagen degradation by pulp fibroblasts.

Wisithphrom K, Murray PE, Windsor LJ
Journal of endodontics 2006 Mar;32(3):186-92

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Metallothionein reduces central nervous system inflammation, neurodegeneration, and cell death following kainic acid-induced epileptic seizures.

Penkowa M, Florit S, Giralt M, Quintana A, Molinero A, Carrasco J, Hidalgo J
Journal of neuroscience research 2005 Feb 15;79(4):522-34

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The in vitro effects of dehydroepiandrosterone on human osteoarthritic chondrocytes.

Jo H, Park JS, Kim EM, Jung MY, Lee SH, Seong SC, Park SC, Kim HJ, Lee MC
Osteoarthritis and cartilage 2003 Aug;11(8):585-94

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The in vitro effects of dehydroepiandrosterone on human osteoarthritic chondrocytes.

Jo H, Park JS, Kim EM, Jung MY, Lee SH, Seong SC, Park SC, Kim HJ, Lee MC
Osteoarthritis and cartilage 2003 Aug;11(8):585-94

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of MMP-3 (Stromelysin-1) using MMP-3 (Stromelysin-1) Monoclonal Antibody (Product # MA5-14210) on Hu Endometrium Cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MMP-3 was performed using tissue extract (30 µg lysate) of Mouse Liver (Lane 1) and 10uL of conditioned media from SK-OV-3 cell line (Lane 2). The blots were probed with Anti-MMP-3 Mouse Monoclonal Antibody (Product # MA5-14210, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 54 kDa band corresponding to MMP-3 was observed in tissue and conditioned media tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of MMP-3 (Stromelysin-1) was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MMP-3 (Stromelysin-1) (SL-1 IID4) Mouse Monoclonal Antibody (Product # MA5-14210) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.