TA321193

antibody from OriGene

Targeting: CHRNA3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA321193

Provider

OriGene

Product name

Anti-CHRNA3 Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to a region derived from 69-83 amino acids of Human cholinergic receptor, nicotinic, alpha 3 (neuronal)

Description

Anti-CHRNA3 Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Concentration

1.2mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 55 kDa . Positive control: Human fetal lung tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CHRNA3 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification: x200)

Validation comment

IHC