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- ELISA [1]
- Immunocytochemistry [2]
- Flow cytometry [2]
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- Product number
- MA5-31685 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHRNA3 Monoclonal Antibody (6D3A10)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-31685 has been tested in indirect ELISA.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6D3A10
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A genetically defined disease model reveals that urothelial cells can initiate divergent bladder cancer phenotypes.
Wang L, Smith BA, Balanis NG, Tsai BL, Nguyen K, Cheng MW, Obusan MB, Esedebe FN, Patel SJ, Zhang H, Clark PM, Sisk AE, Said JW, Huang J, Graeber TG, Witte ON, Chin AI, Park JW
Proceedings of the National Academy of Sciences of the United States of America 2020 Jan 7;117(1):563-572
Proceedings of the National Academy of Sciences of the United States of America 2020 Jan 7;117(1):563-572
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of CHRNA3 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with CHRNA3 monoclonal antibody (Product # MA5-31685) using a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CHRNA3 in Hela cells (green). Sample was incubated with CHRNA3 monoclonal antibody (Product # MA5-31685) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of CHRNA3 in SMMC-7721 cells (green). Sample was incubated with CHRNA3 monoclonal antibody (Product # MA5-31685) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CHRNA3 in SH-SY5Y cells (green). Sample was incubated with CHRNA3 monoclonal antibody (Product # MA5-31685) using a dilution of 1:200-1:400 followed by negative control (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CHRNA3 in SK-N-SH cells (green). Sample was incubated with CHRNA3 monoclonal antibody (Product # MA5-31685) using a dilution of 1:200-1:400 followed by negative control (red).
