ANC-014-200UL

antibody from Invitrogen Antibodies

Targeting: CHRNB4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ANC-014-200UL

Provider

Invitrogen Antibodies

Product name

Nicotinic Acetylcholine Receptor beta 4 (CHRNB4) (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SH-SY5Y human neuroblastoma cell lysate (lanes 1 and 4), rat dorsal root ganglion (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates: - 1-3. Anti-Nicotinic Acetylcholine Receptor beta 4 (CHRNB4) (extracellular) Antibody (#ANC-014), (1:200).4-6. Anti-Nicotinic Acetylcholine Receptor beta 4 (CHRNB4) (extracellular) Antibody , preincubated with Nicotinic Acetylcholine Receptor beta 4/CHRNB4 (extracellular) Blocking Peptide (#BLP-NC014).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SH-SY5Y human neuroblastoma cell lysate (lanes 1 and 4), rat dorsal root ganglion (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates: - 1-3. Anti-Nicotinic Acetylcholine Receptor beta 4 (CHRNB4) (extracellular) Antibody (#ANC-014), (1:200).4-6. Anti-Nicotinic Acetylcholine Receptor beta 4 (CHRNB4) (extracellular) Antibody , preincubated with Nicotinic Acetylcholine Receptor beta 4/CHRNB4 (extracellular) Blocking Peptide (#BLP-NC014).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of nAChR beta 4 in rat PC12 cells - Cell surface detection of nAChR beta 4 in live intact rat pheochromocytoma PC12 cells. A. Cells were stained with Anti-Nicotinic Acetylcholine Receptor beta 4 (CHRNB4) (extracellular) Antibody (#ANC-014), (1:100), followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Merge of the two images.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of nAChR beta 4in rat dorsal root ganglion - Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Nicotinic Acetylcholine Receptor beta 4 (CHRNB4) (extracellular) Antibody (#ANC-014). A. nAChR beta 4 labeling (red) appears in neuronal cell bodies. B. Nuclear staining using DAPI as counterstain (blue). C. Merged images of A and B.