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LearnHPA019587
antibody from Atlas Antibodies
Targeting: UPF1
HUPF1, KIAA0221, NORF1, pNORF1, RENT1, smg-2
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- HPA019587 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA019587, RRID:AB_1856174
- Product name
- Anti-UPF1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human UPF1, Gene description: UPF1 regulator of nonsense transcripts homolog (yeast), Alternative Gene Names: HUPF1, KIAA0221, NORF1, pNORF1, RENT1, smg-2, Validated applications: ICC, IHC, WB, Uniprot ID: Q92900, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references CSL controls telomere maintenance and genome stability in human dermal fibroblasts
Bottoni G, Katarkar A, Tassone B, Ghosh S, Clocchiatti A, Goruppi S, Bordignon P, Jafari P, Tordini F, Lunardi T, Hoetzenecker W, Neel V, Lingner J, Paolo Dotto G
Nature Communications 2019;10(1)
Nature Communications 2019;10(1)
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Enhanced validation
Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting UPF1 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting UPF1 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:114
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-UPF1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
