LS-B15453

antibody from LSBio

Targeting: UPF1 HUPF1, KIAA0221, NORF1, pNORF1, RENT1, smg-2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B15453

Provider

LSBio

Product name

IHC-plus™ RENT1 / UPF1 Antibody (aa1075-C-term) LS-B15453

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Goat

Isotype

IgG

Storage

Store at 2-8°C for up to one year.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human RENT-1 by Western Blot. Samples: Whole cell lysate (30 ug) from lymphoblastoid cell lines (EBV-immortalized B-cell from a normal individual). Antibody: Affinity purified goat anti-RENT-1 antibody used at 1 ug/ml. Detection: Chemiluminescence.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human RENT1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, RKO, MCF-7, Jurkat, TMCK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-RENT1 antibody (lot 4) used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.