RQ5658

antibody from NSJ Bioreagents

Targeting: UPF1 HUPF1, KIAA0221, NORF1, pNORF1, RENT1, smg-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5658

Provider

NSJ Bioreagents

Product name

RENT1 Antibody / hUPF1

Antibody type

Monoclonal

Description

This highly specific RENT1 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

11E7.

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the RENT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HepG2, 2) Raji, 3) PC-3, 4) HeLa and 5) HEK293 lysate with RENT1 antibody. Predicted molecular weight ~124 kDa, but routinely observed at 130-140 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat brain, 2) mouse brain and 3) mouse RAW264.7 lysate with RENT1 antibody. Predicted molecular weight ~124 kDa, but routinely observed at 130-140 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human U-2 OS cells with RENT1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human breast cancer with RENT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse intestine with RENT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse intestine with RENT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat intestine with RENT1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.