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LearnNB100-370
antibody from Novus Biologicals
Targeting: UPF1
HUPF1, KIAA0221, NORF1, pNORF1, RENT1, smg-2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-370 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-370, RRID:AB_10000686
- Product name
- Goat Polyclonal RENT1/UPF1/hUPF1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Saccharomyces cerevisiae Ebs1p is a putative ortholog of human Smg7 and promotes nonsense-mediated mRNA decay.
Luke B, Azzalin CM, Hug N, Deplazes A, Peter M, Lingner J
Nucleic acids research 2007;35(22):7688-97
Nucleic acids research 2007;35(22):7688-97
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Whole cell lysate (30 ug) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody used at 1 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RENT1 antibody (lot For blotting immunoprecipitated RENT1, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RENT1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Detection of human RENT1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, RKO, MCF-7, Jurkat, TMCK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Goat anti-RENT1 antibody NB100-370 used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: RENT1/UPF1/hUPF1 Antibody [NB100-370] - Detection of human RENT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Goat anti-RENT1 antibody NB100-370 (NB100-370 lot 4) used for IP at 6 ug per reaction. RENT1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-370-3). For blotting immunoprecipitated RENT1, NB100-370 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
