AF5209

antibody from Novus Biologicals

Targeting: NDRG1 CAP43, DRG1, NDR1, RTP, TDD5

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5209

Provider

Novus Biologicals

Product name

Goat Polyclonal NDRG1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human NDRG1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human N-myc is observed.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human NDRG1 by Western Blot. Western blot shows lysates of human liver tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NDRG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5209) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NDRG1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.