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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00010397-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010397-M03, RRID:AB_518940
- Product name
- NDRG1 monoclonal antibody (M03), clone 2D7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant NDRG1.
- Antigen sequence
MSREMQDVDLAEVKPLVEKGETITGLLQEFDVQEQ
DIETLHGSVHVTLCGTPKGNRPVILTYHDIGMNHK
TCYNPLFNYEDMQEITQHFAVCHVDAPGQQDGAAS
FPAGYMYPSMDQLAEMLPGVLQQFGLKSIIGMGTG
AGAYILTRFALNNPEMVEGLVLINVNPCAEGWMDW
AASKISGWTQALPDMVVSHLFGKEEMQSNVEVVHT
YRQHIVNDMNPGNLHLFINAYNSRRDLEIERPMPG
THTVTLQCPALLVVGDSSPAVDAVVECNSKLDPTK
TTLLKMADCGGLPQISQPAKLAEAFKYFVQGMGYM
PSASMTRLMRSRTASGSSVTSLDGTRSRSHTSEGT
RSRSHTSEGTRSRSHTSEGAHLDITPNSGAAGNSA
GPKSMEVSC- Isotype
- IgG
- Antibody clone number
- 2D7
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references A novel molecular marker for early detection and evaluating prognosis of gastric cancer: N-myc downstream regulated gene-1 (NDRG1).
Metastasis-related plasma membrane proteins of human breast cancer cells identified by comparative quantitative mass spectrometry.
Jiang K, Shen Z, Ye Y, Yang X, Wang S
Scandinavian journal of gastroenterology 2010 Aug;45(7-8):898-908
Scandinavian journal of gastroenterology 2010 Aug;45(7-8):898-908
Metastasis-related plasma membrane proteins of human breast cancer cells identified by comparative quantitative mass spectrometry.
Leth-Larsen R, Lund R, Hansen HV, Laenkholm AV, Tarin D, Jensen ON, Ditzel HJ
Molecular & cellular proteomics : MCP 2009 Jun;8(6):1436-49
Molecular & cellular proteomics : MCP 2009 Jun;8(6):1436-49
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged NDRG1 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to NDRG1 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to NDRG1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 0.7 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
