LS-C818174

antibody from LSBio

Targeting: NDRG1 CAP43, DRG1, NDR1, RTP, TDD5

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C818174

Provider

LSBio

Product name

NDRG1 Antibody (Preservative Free) LS-C818174

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

NDRG1 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 2 uL anti-NDRG1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NDRG1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

NDRG1 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 2 uL anti-NDRG1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NDRG1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 43 kDa.