Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP2-27175 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal AG-2/AGR2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Bovine, Canine, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: AG-2 Antibody (10E2) [NBP2-27175] - Analysis of human stomach in the A) absense and B) presence of immunizing peptide, C) mouse stomach, D) rat stomach tissue lysate, and E) HCT-116 cell lysate using AGR2 antibody at 5 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - IHC-P detection of AGR2 protein in a human ovarian cancer section using 5 ug/ml concentration of AGR2 antibody. The representative image shows AGR2 immuno-reactivity in the developing ovarian cancer and the follicle cells of Graafian follicles. The cells of medulla region in the ovary did not show AGR2 positivity. [10X magnification]
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - IHC-P detection of AGR2 protein in a section of human bladded cancer using 5 ug/ml concentration of AGR2 antibody. The AGR2 immunoreactivity was observed in cellular cytoplasm and the inter-cellular spaces in cancerous areas of the sections. [40X magnification]
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - Formalin-fixed, paraffin-embedded human breast stained with AGR2 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - Paraffin-embedded human small intestine tissue stained with AGR2 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) [NBP2-27175] - Formalin-fixed, paraffin-embedded human spleen tissue stained with 5 ug/ml concentration of AGR2 antibody .