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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [14]
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- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- NBP2-27393 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal AG-2/AGR2 Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified. Researchers are encouraged to use the sequence and accession number (NP_006399.1) that were used to design the peptide immunogen in order to assess potential cross-reactivity of the antibody to their species of interest.The AGR2 antibody is specific to AGR2 and does not recognize AGR3 (Verma et al, 2012)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Single-cell RNA sequencing demonstrates the molecular and cellular reprogramming of metastatic lung adenocarcinoma.
Genomic Network-Based Analysis Reveals Pancreatic Adenocarcinoma Up-Regulating Factor-Related Prognostic Markers in Cervical Carcinoma.
Clinicopathological and prognostic significance of MUC13 and AGR2 expression in intraductal papillary mucinous neoplasms of the pancreas.
ER stress protein AGR2 precedes and is involved in the regulation of pancreatic cancer initiation.
Anterior gradient 2 downregulation in a subset of pancreatic ductal adenocarcinoma is a prognostic factor indicative of epithelial-mesenchymal transition.
Knockdown of anterior gradient 2 expression extenuates tumor-associated phenotypes of SNU-478 ampulla of Vater cancer cells.
Loss of anterior gradient 2 (Agr2) expression results in hyperplasia and defective lineage maturation in the murine stomach.
AGR2 is a SMAD4-suppressible gene that modulates MUC1 levels and promotes the initiation and progression of pancreatic intraepithelial neoplasia.
The estrogen-responsive Agr2 gene regulates mammary epithelial proliferation and facilitates lobuloalveolar development.
SPDEF is required for mouse pulmonary goblet cell differentiation and regulates a network of genes associated with mucus production.
Analysis of the pancreatic tumor progression by a quantitative proteomic approach and immunhistochemical validation.
The protein disulfide isomerase AGR2 is essential for production of intestinal mucus.
The adenocarcinoma-associated antigen, AGR2, promotes tumor growth, cell migration, and cellular transformation.
Proteomic changes during intestinal cell maturation in vivo.
Kim N, Kim HK, Lee K, Hong Y, Cho JH, Choi JW, Lee JI, Suh YL, Ku BM, Eum HH, Choi S, Choi YL, Joung JG, Park WY, Jung HA, Sun JM, Lee SH, Ahn JS, Park K, Ahn MJ, Lee HO
Nature communications 2020 May 8;11(1):2285
Nature communications 2020 May 8;11(1):2285
Genomic Network-Based Analysis Reveals Pancreatic Adenocarcinoma Up-Regulating Factor-Related Prognostic Markers in Cervical Carcinoma.
Kim J, Chung JY, Kim TJ, Lee JW, Kim BG, Bae DS, Choi CH, Hewitt SM
Frontiers in oncology 2018;8:465
Frontiers in oncology 2018;8:465
Clinicopathological and prognostic significance of MUC13 and AGR2 expression in intraductal papillary mucinous neoplasms of the pancreas.
Mito K, Saito M, Morita K, Maetani I, Sata N, Mieno M, Fukushima N
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 2018 Jun;18(4):407-412
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 2018 Jun;18(4):407-412
ER stress protein AGR2 precedes and is involved in the regulation of pancreatic cancer initiation.
Dumartin L, Alrawashdeh W, Trabulo SM, Radon TP, Steiger K, Feakins RM, di Magliano MP, Heeschen C, Esposito I, Lemoine NR, Crnogorac-Jurcevic T
Oncogene 2017 Jun 1;36(22):3094-3103
Oncogene 2017 Jun 1;36(22):3094-3103
Anterior gradient 2 downregulation in a subset of pancreatic ductal adenocarcinoma is a prognostic factor indicative of epithelial-mesenchymal transition.
Mizuuchi Y, Aishima S, Ohuchida K, Shindo K, Fujino M, Hattori M, Miyazaki T, Mizumoto K, Tanaka M, Oda Y
Laboratory investigation; a journal of technical methods and pathology 2015 Feb;95(2):193-206
Laboratory investigation; a journal of technical methods and pathology 2015 Feb;95(2):193-206
Knockdown of anterior gradient 2 expression extenuates tumor-associated phenotypes of SNU-478 ampulla of Vater cancer cells.
Kim SJ, Jun S, Cho HY, Lee DC, Yeom YI, Kim JH, Kang D
BMC cancer 2014 Nov 3;14:804
BMC cancer 2014 Nov 3;14:804
Loss of anterior gradient 2 (Agr2) expression results in hyperplasia and defective lineage maturation in the murine stomach.
Gupta A, Wodziak D, Tun M, Bouley DM, Lowe AW
The Journal of biological chemistry 2013 Feb 8;288(6):4321-33
The Journal of biological chemistry 2013 Feb 8;288(6):4321-33
AGR2 is a SMAD4-suppressible gene that modulates MUC1 levels and promotes the initiation and progression of pancreatic intraepithelial neoplasia.
Norris AM, Gore A, Balboni A, Young A, Longnecker DS, Korc M
Oncogene 2013 Aug 15;32(33):3867-76
Oncogene 2013 Aug 15;32(33):3867-76
The estrogen-responsive Agr2 gene regulates mammary epithelial proliferation and facilitates lobuloalveolar development.
Verma S, Salmans ML, Geyfman M, Wang H, Yu Z, Lu Z, Zhao F, Lipkin SM, Andersen B
Developmental biology 2012 Sep 15;369(2):249-60
Developmental biology 2012 Sep 15;369(2):249-60
SPDEF is required for mouse pulmonary goblet cell differentiation and regulates a network of genes associated with mucus production.
Chen G, Korfhagen TR, Xu Y, Kitzmiller J, Wert SE, Maeda Y, Gregorieff A, Clevers H, Whitsett JA
The Journal of clinical investigation 2009 Oct;119(10):2914-24
The Journal of clinical investigation 2009 Oct;119(10):2914-24
Analysis of the pancreatic tumor progression by a quantitative proteomic approach and immunhistochemical validation.
Sitek B, Sipos B, Alkatout I, Poschmann G, Stephan C, Schulenborg T, Marcus K, Lüttges J, Dittert DD, Baretton G, Schmiegel W, Hahn SA, Klöppel G, Meyer HE, Stühler K
Journal of proteome research 2009 Apr;8(4):1647-56
Journal of proteome research 2009 Apr;8(4):1647-56
The protein disulfide isomerase AGR2 is essential for production of intestinal mucus.
Park SW, Zhen G, Verhaeghe C, Nakagami Y, Nguyenvu LT, Barczak AJ, Killeen N, Erle DJ
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 28;106(17):6950-5
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 28;106(17):6950-5
The adenocarcinoma-associated antigen, AGR2, promotes tumor growth, cell migration, and cellular transformation.
Wang Z, Hao Y, Lowe AW
Cancer research 2008 Jan 15;68(2):492-7
Cancer research 2008 Jan 15;68(2):492-7
Proteomic changes during intestinal cell maturation in vivo.
Chang J, Chance MR, Nicholas C, Ahmed N, Guilmeau S, Flandez M, Wang D, Byun DS, Nasser S, Albanese JM, Corner GA, Heerdt BG, Wilson AJ, Augenlicht LH, Mariadason JM
Journal of proteomics 2008 Dec 2;71(5):530-46
Journal of proteomics 2008 Dec 2;71(5):530-46
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: AG-2/AGR2 Antibody [NBP2-27393] - Analysis using the Azide Free version of NBP2-27393. Simple Western lane view shows a specific band for AGR2 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AG-2/AGR2 Antibody [NBP2-27393] - AGR2 expression in cancer cell lines of the biliary tract. AGR2 mRNA levels in six biliary tract cancer cell lines were measured by real-time RT-PCR. The AGR2 transcript levels were normalized against those of the GAPDH. Results shown are means +/- standard deviations of (1/2)CT of target - CT of GAPDH of three experiments. B. AGR2 protein expression was detected by western blot analysis with beta-actin as a loading control. The result shown is a representative of three experiments. Abbreviations: CBD, common bile duct; GB, gall bladder; AoV, ampulla of Vater; IHD, intrahepatic duct; HDB, hepatic duct bifurcation; WD, well differentiated; MD, moderately differentiated; PD, poorly differentiated [24]. Image collected and cropped by CiteAb from the following publication (http://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-804), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AG-2/AGR2 Antibody [NBP2-27393] - Analysis using the Azide Free version of NBP2-27393. Detection of AGR2 using AGR2 antibody. MCF7 (A), Caco-2 (B) and human stomach lysate (C) probed with AGR2 antibody at 1:100. were used for this test. The higher molecular weight band of variable intensity at ~30 kDa is uncharacterized and may represent a form of AGR2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AG-2 Antibody [NBP2-27393] - Analysis using the Azide Free version of NBP2-27393. Staining of AGR2 using AGR2 antibody. Staining of normal colon tissue probed with AGR2 antibody at 1:100 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody [NBP2-27393] - Analysis using the Azide Free version of NBP2-27393. Staining of human adenocarcinoma of the breast stained with AGR2 antibody (2 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AG-2/AGR2 Antibody [NBP2-27393] - Analysis using the Azide Free version of NBP2-27393. Staining of human adenocarcinoma of the colon stained with AGR2 antibody (2-5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
