AP6279c

antibody from Abgent

Targeting: AGR2 AG2, HAG-2, PDIA17, XAG-2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP6279c

Provider

Abgent

Proper citation

Abgent Cat#AP6279c, RRID:AB_2258115

Product name

AGR2 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Western blot analysis of lysates from ZR-75-1, HT29 cell line (from left to right), using AGR2 Antibody (Center)(Cat. #AP6279c). AP6279c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane."

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"AGR2 Antibody (Center) (Cat. #AP6279c) western blot analysis in HT29,MCF-7,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the AGR2 antibody detected the AGR2 protein (arrow)."

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50