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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX81925 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81925, RRID:AB_11176437
- Product name
- Anterior Gradient 2 antibody, N-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of AGR2 Antibody (N-term)(GTX81925) in T47D cell line lysates (35μg/lane). AGR2 (arrow) was detected using the purified Pab.
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- GeneTex (provider)
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- Experimental details
- Western blot analysis of AGR2(arrow) using rabbit polyclonal AGR2 Antibody (N-term) (GTX81925).293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected with the AGR2 gene (Lane 2)
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Fluorescent confocal image of A549 cell stained with AGR2 Antibody (N-term)(GTX81925).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37¢J). For secondary antibody, Alexa Fluor? 488 conjμgated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37¢J).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjμgated Phalloidin (7units/ml, 1 h at 37¢J). Nuclei were counterstained with DAPI (blue) (10 μg/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (N-term) (GTX81925), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
