NBP1-89212

antibody from Novus Biologicals

Targeting: AGR2 AG2, HAG-2, PDIA17, XAG-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-89212

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-89212, RRID:AB_11024182

Product name

Rabbit Polyclonal AG-2/AGR2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human AG-2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

AGR2 predicts tamoxifen resistance in postmenopausal breast cancer patients.

Hrstka R, Brychtova V, Fabian P, Vojtesek B, Svoboda M
Disease markers 2013;35(4):207-12

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Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.

Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: AG-2 Antibody [NBP1-89212] - Analysis in human cell lines A-549 and HEK293 using anti-AGR2 antibody. Corresponding AGR2 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Staining of human liver shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Staining of human rectum shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Staining in human rectum and liver tissues using anti-AGR2 antibody. Corresponding AGR2 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Staining of human stomach shows moderate to strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Analysis in human stomach and skeletal muscle tissues. Corresponding AGR2 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Staining of human endometrium shows moderate to strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AG-2 Antibody [NBP1-89212] - Staining of human skeletal muscle shows no positivity in myocytes as expected.